Barid Izzata, Nawawi Soetomo, Sosroseno Wihaskoro
Department of Oral Biology and Conservative Dentistry, University of Jember, Indonesia.
J Endod. 2002 Dec;28(12):806-10. doi: 10.1097/00004770-200212000-00004.
The aim of this study was to determine whether the phagocytic functions of a murine macrophage cell line (RAW264.7 cells) may be altered by parachlorophenol (PCP) and camphorated parachlorophenol (CMCP). The adherence capacities of PCP- or CMCP-treated cells on plastic surfaces were much lower than those of untreated cells. When the PCP- or CMCP-treated cells were incubated with Actinobacillus actinomycetemcomitans, phagocytosis of these cells was significantly reduced in a dose-dependent fashion compared with that of untreated cells. Preactivation with bacterial lipopolysaccharide on PCP- or CMCP-treated cells failed to completely restore the phagocytosis of this periodontopathogen. The phagocytic functions of PCP- or CMCP-treated cells to this periodontopathogen opsonized with anti-A. actinomycetemcomitans were also reduced compared with those of untreated cells but were comparable with those of untreated cells incubated with unopsonized bacteria. The results of this study indicated, therefore, that both PCP and CMCP may, indeed, reduce the phagocytic activities of murine macrophages.
本研究的目的是确定对氯苯酚（PCP）和樟脑对氯苯酚（CMCP）是否会改变小鼠巨噬细胞系（RAW264.7细胞）的吞噬功能。PCP或CMCP处理的细胞在塑料表面的黏附能力远低于未处理的细胞。当PCP或CMCP处理的细胞与伴放线放线杆菌一起孵育时，与未处理的细胞相比，这些细胞的吞噬作用以剂量依赖的方式显著降低。用细菌脂多糖对PCP或CMCP处理的细胞进行预激活未能完全恢复对这种牙周病原体的吞噬作用。与未处理的细胞相比，PCP或CMCP处理的细胞对用抗伴放线放线杆菌调理的这种牙周病原体的吞噬功能也降低了，但与用未调理细菌孵育的未处理细胞的吞噬功能相当。因此，本研究结果表明，PCP和CMCP确实都可能降低小鼠巨噬细胞的吞噬活性。
