Messer H H, Feigal R J
J Dent Res. 1985 May;64(5):818-21. doi: 10.1177/00220345850640050701.
The antibacterial and cytotoxic effects of parachlorophenol (as 35% camphorated PCP and 2% aqueous PCP) were compared directly, using the agar overlay technique. Human pulp fibroblasts and L929 cells were grown to confluence in 60-mm petri dishes. Bacterial suspensions (S. aureus, E. coli) in agar were poured as a thin layer in 60-mm petri dishes. All were overlaid with agar. Cells were exposed to medicament via filter paper discs placed on the agar surface. After 24 hours, the zone of cell lysis (fibroblasts) or inhibition of bacterial growth was measured as diameter in mm. A dose-response relationship was observed for both fibroblasts and bacteria. The zone of inhibition for fibroblasts was larger than that for bacteria, indicating that the cytotoxicity of parachlorophenol exceeds its antibacterial activity.
采用琼脂覆盖技术直接比较了对氯苯酚（以35%樟脑对氯苯酚和2%对氯苯酚水溶液形式）的抗菌和细胞毒性作用。人牙髓成纤维细胞和L929细胞在60毫米培养皿中生长至汇合。将含有细菌悬液（金黄色葡萄球菌、大肠杆菌）的琼脂作为薄层倒入60毫米培养皿中。所有培养皿都覆盖上琼脂。通过放置在琼脂表面的滤纸圆盘使细胞接触药物。24小时后，测量细胞裂解区（成纤维细胞）或细菌生长抑制区的直径（以毫米为单位）。在成纤维细胞和细菌中均观察到剂量反应关系。成纤维细胞的抑制区大于细菌的抑制区，表明对氯苯酚的细胞毒性超过其抗菌活性。
