Neff Theodore, Layman Don, Jeansonne Billie Gail
Department of Endodontics, Louisiana State University Health Sciences Center, School of Dentistry, New Orleans 70119, USA.
J Endod. 2002 Dec;28(12):811-4. doi: 10.1097/00004770-200212000-00005.
During warm-vertical condensation of gutta-percha, endodontic sealers are exposed to temperatures that potentially alter their cytotoxicity profiles. This study investigated the effects of heat on sealer toxicity by using the CyQuant assays for cell adhesion and proliferation of human gingival fibroblasts. Seven sealers were exposed to one of the following temperatures for 10 min before assay: 23 degrees C, 43 degrees C, or 100 degrees C. Adhesion data were used to determine percentage of cells bound, and proliferation data were used to determine percentage of change in cell number from 24 to 72 h, for each sealer at each temperature. The temperatures of 23 degrees C, 43 degrees C, and 100 degrees C were significantly different in both the adhesion and proliferation assays. Sealer type and temperature were significant variables in both assays. The changes in toxicity measured by adhesion and proliferation for each sealer were not always similar. Roth 801 and Roth 811, after exposure to 100 degrees C, showed enhanced adhesion but not proliferation, whereas Kerr PCS and Dentalis, after exposure to 100 degrees C, showed decreased adhesion but enhanced proliferation. AH Plus and AH26 showed an increase in adhesion and proliferation after heat exposure, whereas Sealapex remained virtually unaffected.
在热牙胶垂直冷凝过程中，根管封闭剂会暴露于可能改变其细胞毒性特征的温度环境中。本研究通过使用CyQuant分析法检测人牙龈成纤维细胞的黏附和增殖情况，来探究加热对封闭剂毒性的影响。在检测前，将七种封闭剂分别置于以下温度之一达10分钟：23摄氏度、43摄氏度或100摄氏度。黏附数据用于确定细胞黏附百分比，增殖数据用于确定每种封闭剂在每个温度下从24小时到72小时细胞数量的变化百分比。在黏附和增殖检测中，23摄氏度、43摄氏度和100摄氏度这三个温度存在显著差异。封闭剂类型和温度在两种检测中均为显著变量。每种封闭剂通过黏附和增殖检测所测得的毒性变化并不总是相似的。Roth 801和Roth 811在暴露于100摄氏度后，黏附增强但增殖未增强，而Kerr PCS和Dentalis在暴露于100摄氏度后，黏附降低但增殖增强。AH Plus和AH26在热暴露后黏附和增殖均增加，而Sealapex几乎未受影响。
