Department of Operative Dentistry, University Medical Centre of the Johannes Gutenberg University Mainz, Germany.
Head Face Med. 2011 Aug 10;7:15. doi: 10.1186/1746-160X-7-15.
The aim of this study was to investigate in vitro the cytotoxicity and antibacterial properties of four different endodontic sealers using human periodontal ligament fibroblast cell proliferation and visual analysis of growth inhibition.
A silicone (GuttaFlow), silicate (EndoSequence BC), zinc oxide eugenol (Pulp Canal Sealer EWT) and epoxy resin (AH Plus Jet) based sealer were incubated with PDL fibroblasts (10⁴ cells/ml, n=6) up to 96 h. Cell proliferation (RFU) was determined by means of the Alamar Blue assay. Cell growth and morphology was visualized by means of fluorescent dyes. Possible antibacterial properties of the different sealers were visualized by means of SEM (Enterococcus faecalis; Parvimonas micra).
Fibroblast proliferation depended on sealer and cultivation time. After 72 and 96 h GuttaFlow and EndoSequence BC showed relatively non-cytotoxic reactions, while Pulp Canal Sealer EWT and AH Plus Jet caused a significant decrease of cell proliferation (p<0.001). Visualization of cell growth and morphology with various fluorescent dyes supplemented the results. No antibacterial effect of EndoSequence BC to P. micra was found, whereas GuttaFlow showed a weak, Pulp Canal Sealer EWT and AH Plus Jet extensive growth inhibition. Also, no antibacterial effect of GuttaFlow, EndoSequence BC or AH Plus Jet to E. faecalis could be detected.
These in vitro findings reveal that GuttaFlow and EndoSequence BC can be considered as biocompatible sealing materials. However, prior to their clinical employment, studies regarding their sealing properties also need to be considered.
本研究旨在通过观察人牙周膜成纤维细胞生长抑制的视觉分析和增殖情况，体外研究 4 种不同根管封闭剂的细胞毒性和抗菌性能。
将一种硅胶（GuttaFlow）、硅酸盐水基（EndoSequence BC）、氧化锌丁香酚（Pulp Canal Sealer EWT）和环氧树脂（AH Plus Jet）基封闭剂与牙周膜成纤维细胞（10⁴个细胞/ml，n=6）孵育长达 96 小时。采用 Alamar Blue 测定法测定细胞增殖（相对荧光单位，RFU）。通过荧光染料观察细胞生长和形态。通过扫描电子显微镜（Enterococcus faecalis；Parvimonas micra）观察不同封闭剂的可能抗菌性能。
成纤维细胞的增殖取决于封闭剂和培养时间。GuttaFlow 和 EndoSequence BC 在 72 和 96 小时后显示出相对非细胞毒性反应，而 Pulp Canal Sealer EWT 和 AH Plus Jet 则导致细胞增殖显著减少（p<0.001）。使用各种荧光染料观察细胞生长和形态补充了结果。未发现 EndoSequence BC 对 P. micra 有抗菌作用，而 GuttaFlow 显示出较弱的作用，Pulp Canal Sealer EWT 和 AH Plus Jet 则显示出广泛的生长抑制作用。此外，也未检测到 GuttaFlow、EndoSequence BC 或 AH Plus Jet 对 E. faecalis 的抗菌作用。
这些体外研究结果表明，GuttaFlow 和 EndoSequence BC 可被视为生物相容性的封闭材料。然而，在将其临床应用之前，还需要考虑研究其封闭性能。
