Cieri U R
J Assoc Off Anal Chem. 1976 Jan;59(1):60-1.
The animal feed sample is extracted with alcohol, the extract is evaporated to a small volume, and an aliquot is spotted on a silica gel H plate. After development of the plate in chloroform-methanol (80+20), the spotting area is scraped and extracted with ethanol, which is evaporated to dryness. The residue is dissolved in 3 ml alcohol, and an aliquot is reacted with p-dimethylaminobenzaldehyde solution; another aliquot of the extract is used as a sample blank. A control is prepared by spiking another portion of the same feed with about 5 ppm arsanilic acid. The method is applicable in the presence of procaine and sulfonamides.
将动物饲料样品用酒精提取，提取物蒸发至小体积，取一份点样于硅胶H板上。在氯仿 - 甲醇（80 + 20）中展开薄板后，刮下点样区域并用乙醇提取，然后蒸发至干。残留物溶于3毫升酒精中，取一份与对二甲氨基苯甲醛溶液反应；提取物的另一份作为样品空白。通过在另一份相同饲料中加入约5 ppm的对氨基苯磺酸来制备对照品。该方法适用于存在普鲁卡因和磺胺类药物的情况。
