Su Bao-Ning, Cuendet Muriel, Farnsworth Norman R, Fong Harry H S, Pezzuto John M, Kinghorn A Douglas
Program for Collaborative Research in the Pharmaceutical Sciences and Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA.
Planta Med. 2002 Dec;68(12):1125-8. doi: 10.1055/s-2002-36354.
Bioactivity-guided fractionation of petroleum ether- and EtOAc-soluble extracts of the seeds of Ziziphus jujuba using a cyclooxygenase-2 assay as a monitor indicated that the triglyceride, 1,3-di-O-[9(Z)-octadecenoyl]-2-O-[9(Z),12(Z)-octadecadienoyl]glycerol (3), and a fatty acid mixture of linoleic, oleic and stearic acids, were the major active components. A new pentacyclic lupane-type triterpene derivative, 3-O-[9(Z)-octadecenoyl]betulinic acid (1), and betulinic acid (2) were also isolated and identified. All isolates as well as pure linoleic, oleic and stearic acids were evaluated for their inhibitory effects against both cyclooxygenases-1 (COX-1) and -2 (COX-2).
以环氧合酶-2分析为监测手段，对酸枣种子石油醚和乙酸乙酯可溶提取物进行生物活性导向分离，结果表明甘油三酯、1,3-二-O-[9(Z)-十八碳烯酰基]-2-O-[9(Z),12(Z)-十八碳二烯酰基]甘油（3）以及亚油酸、油酸和硬脂酸的脂肪酸混合物是主要活性成分。还分离并鉴定出一种新的五环羽扇豆烷型三萜衍生物3-O-[9(Z)-十八碳烯酰基]桦木酸（1）和桦木酸（2）。对所有分离物以及纯亚油酸、油酸和硬脂酸进行了对环氧合酶-1（COX-1）和-2（COX-2）的抑制作用评估。
