Sela B A, Wang J L, Edelman G M
J Exp Med. 1976 Mar 1;143(3):665-71. doi: 10.1084/jem.143.3.665.
Antibodies reactive with cell surface carbohydrates were isolated from normal chicken serum and were found to be mitogenic for mouse splenic lymphocytes as assayed by both blast transformation and [3H]thymidine incorporation. The Fab' fragments of these carbohydrate-binding immunoglobulins were just as mitogenic as the divalent native antibody. Moreover, succinylated Fab' fragments, which probably would not form self-associating aggregates, showed similar mitogenic properties. All of these results indicate that, at least for saccharide-specific ligands, multipoint attachment and receptor cross-linkage on the cell to which the ligand is attached may not be a stringent requirement for activation.
从正常鸡血清中分离出与细胞表面碳水化合物发生反应的抗体，通过细胞增殖转化和[3H]胸腺嘧啶核苷掺入法检测发现，这些抗体对小鼠脾淋巴细胞具有促有丝分裂作用。这些碳水化合物结合免疫球蛋白的Fab'片段与二价天然抗体一样具有促有丝分裂作用。此外，可能不会形成自缔合聚集体的琥珀酰化Fab'片段也表现出类似的促有丝分裂特性。所有这些结果表明，至少对于糖类特异性配体而言，配体所附着细胞上的多点附着和受体交联可能不是激活的严格要求。
