Zhang T, Gong W, Li Z, Yang S, Zhang K, Yin D, Xu P, Jia T
Shandong University Medical School, Wenhua West Road 44, Jinan, PR China 250012.
Undersea Hyperb Med. 2007 Sep-Oct;34(5):335-9.
This study was designed to determine if hyperbaric oxygen improved random pattern skin flap survival in diabetic rats.
Cranially-based, 4 x 10 cm dorsal skin flaps were raised in 38 diabetic rats induced by streptozocin (STZ). The animals were randomly divided equally into two groups. Group A was a control group observed in the room air and Group B was the experimental group, which received hyperbaric oxygen (HBO2) therapy. The HBO2 regimen consisted of 90 minutes of treatment with 100% O2 at 2.5 ATA (atmosphere absolute ATA) per day for 7 consecutive days. On the 7th postoperative day, we measured the necrotic flap area and the new growth number of capillary vessel and the granulation tissue thickness.
The percentage of necrosis flap area for group A was 50.5 +/- 10.5%; for group B it was 38.5 +/- 9.3%. The reduction in necrosis flap area was highly significant (p < 0.01) compared with controls. Also, new-growth capillary vessel and granulation tissue thickness were statistically different between the two groups.
The findings of this study demonstrated beneficial effects of HBO2 in improving diabetic rat dorsal skin flap survival.
本研究旨在确定高压氧是否能提高糖尿病大鼠随意型皮瓣的存活率。
对38只由链脲佐菌素（STZ）诱导的糖尿病大鼠制作基于颅骨的4×10 cm背部皮瓣。将动物随机均分为两组。A组为在室内空气中观察的对照组，B组为接受高压氧（HBO2）治疗的实验组。HBO2治疗方案为每天在2.5个绝对大气压（ATA）下用100%氧气治疗90分钟，连续7天。术后第7天，测量皮瓣坏死面积、新生毛细血管数量和肉芽组织厚度。
A组皮瓣坏死面积百分比为50.5±10.5%；B组为38.5±9.3%。与对照组相比，皮瓣坏死面积的减少非常显著（p<0.01）。此外，两组之间新生毛细血管和肉芽组织厚度在统计学上存在差异。
本研究结果表明高压氧对提高糖尿病大鼠背部皮瓣存活率有有益作用。
