Oh G S, Pae H O, Choi B M, Jeong S, Oh H, Oh C S, Rho Y D, Kim D H, Shin M K, Chung Hun-Taeg
Department of Microbiology and Immunology, Medicinal Resources Research Center of Wonkwang University, Wonkwang University School of Medicine, 344-2 Shinyong-Dong, Iksan-Shi, Chonbuk 570-749, South Korea.
J Ethnopharmacol. 2003 Jan;84(1):85-9. doi: 10.1016/s0378-8741(02)00285-4.
In an effort to elucidate the mechanism of the anti-inflammatory effect of mudanpi, the root cortex of Paeonia suffruticosa Andrews (Ranunculaceae), we determined the effects of the methanolic extract of mudanpi (MEM) on the secretions of interleukin (IL)-8, a major mediator of acute neutrophil-mediated inflammation, and macrophage chemoattractant protein (MCP)-1, a major mediator of chronic macrophage-mediated inflammation, in human monocytic U937 cells stimulated with phorbol myristate acetate (PMA). MEM significantly inhibited PMA-induced secretions of IL-8 and MCP-1 proteins in a dose-dependent manner. The inhibition of these chemokines by MEM was due to its suppression of IL-8 and MCP-1 genes. In addition, 1,2,3,4,6-penta-O-galloyl-beta-D-glucose, one of major constituents isolated from MEM, inhibited PMA-induced secretions of IL-8 and MCP-1 proteins by its suppression of IL-8 and MCP-1 genes. Thus, one possible anti-inflammatory mechanism of mudanpi, an anti-inflammatory Chinese crude drug, may be to inhibit the secretions of inflammatory chemokines.
