Soto S M, Lobato M J, Mendoza M C
Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006-Oviedo, Spain.
Antimicrob Agents Chemother. 2003 Jan;47(1):421-6. doi: 10.1128/AAC.47.1.421-425.2003.
Seventy nine strains of Yersinia enterocolitica resistant to one or more antimicrobials were analyzed for integrons. Only class 1 sul1 integrons containing aadA1a (28 strains), aadA1a-dfr1-sat1 (2 strains), and dfr1-aadA1a (1 strain) gene cassettes were found. The first two types were found in clinical isolates belonging to serotype O:3, biotypes 2 to 4, and eight combined ribotypes, and the third was found in the reference strain, CECT4054 (O:8). All screened resistance markers were found in strains with and without integrons (except for chloramphenicol resistance, encoded by catA1 gene, which was only present in strains with integrons), but in different resistance profiles (R profiles). A profile (ampicillin, streptomycin, sulfadiazine, and trimethoprim resistance, encoded by the tem1, aadA1a, sul1, and dfr1 genes, respectively) was found in strains, with and without integrons. Integrons and some of the resistance genes are located on plasmids with sizes ranging between 65 and 140 kb. This is the first report of class 1 integrons in Y. enterocolitica.
对79株对一种或多种抗菌药物耐药的小肠结肠炎耶尔森菌进行了整合子分析。仅发现了含有aadA1a（28株）、aadA1a-dfr1-sat1（2株）和dfr1-aadA1a（1株）基因盒的1类sul1整合子。前两种类型存在于血清型为O:3、生物型为2至4且有8种合并核糖体分型的临床分离株中，第三种类型存在于参考菌株CECT4054（O:8）中。所有筛选的耐药标记在有整合子和无整合子的菌株中均有发现（除了由catA1基因编码的氯霉素耐药性，仅存在于有整合子的菌株中），但耐药谱（R谱）不同。在有整合子和无整合子的菌株中均发现了一种谱型（分别由tem1、aadA1a、sul1和dfr1基因编码的氨苄西林、链霉素、磺胺嘧啶和甲氧苄啶耐药性）。整合子和一些耐药基因位于大小在65至140 kb之间的质粒上。这是小肠结肠炎耶尔森菌中1类整合子的首次报道。
