与阿尔茨海默病βA4-淀粉样前体蛋白相关的精子膜蛋白YWK-II/APPH部分细胞外片段的结晶及初步晶体学分析
Crystallization and preliminary crystallographic analysis of a partial extracellular fragment of a sperm membrane protein YWK-II/APPH related to the Alzheimer betaA4-amyloid precursor protein.
作者信息
Yang Maojun, Liu Binbin, Zhang Xiaodong, Zhou Weihong, Gao Feng, Pang Hai, Miao Shiying, Wang Linfang, Rao Zihe
机构信息
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People's Republic of China.
出版信息
Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):146-9. doi: 10.1107/s0907444902018024. Epub 2002 Dec 19.
Crystals of a partial extracellular fragment of a human sperm membrane protein YWK-II/APPH have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.8 A resolution at 100 K using Cu K(alpha) radiation. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 46.009, b = 67.387, c = 149.241 A, alpha = beta = gamma = 90 degrees. The presence of two molecules per asymmetric unit gives a crystal Volume per protein mass (V(M)) of 3.51 A(3) Da(-1) and a solvent content of 64.6% by Volume. A full set of X-ray diffraction data were collected to 2.8 A resolution from the native crystal.
利用聚乙二醇4000作为沉淀剂，在291 K温度下培养出了人精子膜蛋白YWK-II/APPH部分细胞外片段的晶体。使用铜Kα辐射，该晶体在100 K温度下的衍射图谱分辨率达到2.8 Å。晶体属于空间群P2(1)2(1)2(1)，晶胞参数为a = 46.009、b = 67.387、c = 149.241 Å，α = β = γ = 90°。每个不对称单元中有两个分子，晶体的每蛋白质量晶体体积（V(M)）为3.51 ų Da⁻¹，溶剂含量为64.6%（体积）。从天然晶体收集了一套完整的分辨率为2.8 Å的X射线衍射数据。
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