Maoka Takashi, Tsushima Miyuki, Nishino Hoyoku
Kyoto Pharamceutical University, Kyoto, Japan.
Chem Pharm Bull (Tokyo). 2002 Dec;50(12):1630-3. doi: 10.1248/cpb.50.1630.
Two epimeric carotenoids, named dinochromes A (2) and B (3), were isolated from the fresh water red tide Peridinium bipes, as anti-carcinogenic compounds. The stereostructure of dinochrome A and B were characterized to be (3S,5R,6R,3'S,5'R,8'R)- and (3S,5R,6R,3'S,5'R,8'S)-5',8'-epoxy-6,7-didehydro-5,6,5',8'-tetrahydro-beta,beta-carotene-3,5,3'-triol 3-O-acetate, respectively by (1)H- and (13)C-NMR, and circular dichroism (CD) data. Dinochromes A (2) and B (3) inhibit 12-O-tetradecanoyl phorbol 13-acetate (TPA)-stimulated (32)P-incorporation into the phosholipids of HeLa cells. Furthermore, dinochrome A was found to inhibit the proliferation of human malignant tumor cells, such as GOTO, OST and HeLa cells.
从淡水赤潮双足多甲藻中分离出两种差向异构类胡萝卜素，命名为恐龙色素A（2）和B（3），作为抗癌化合物。通过¹H-和¹³C-NMR以及圆二色性（CD）数据，确定恐龙色素A和B的立体结构分别为（3S,5R,6R,3'S,5'R,8'R）-和（3S,5R,6R,3'S,5'R,8'S）-5',8'-环氧-6,7-二脱氢-5,6,5',8'-四氢-β,β-胡萝卜素-3,5,3'-三醇3-O-乙酸酯。恐龙色素A（2）和B（3）可抑制12-O-十四烷酰佛波醇13-乙酸酯（TPA）刺激的³²P掺入HeLa细胞的磷脂中。此外，发现恐龙色素A可抑制人恶性肿瘤细胞如GOTO、OST和HeLa细胞的增殖。
