Anzai Toru, Wada Ruichi, Okuda Toshio, Aoki Toshihisa
Epizootic Research Station, Equine Research Institute, Japan Racing Association, Tochigi, Japan.
J Vet Med Sci. 2002 Nov;64(11):999-1002. doi: 10.1292/jvms.64.999.
The effectiveness of the polymerase chain reaction (PCR) as a field application test for the eradication of contagious equine metritis (CEM) was evaluated. Seven-thousands five-hundred and thirty-four genital swabs were collected from 4,026 Thoroughbred broodmares and stallions in Japan to test "high risk" horses as well as for general surveillance testing from 1998 to 2001. Bacterial isolation as well as PCR testing of original specimens and cultured specimens was performed for detection of Taylorella equigenitalis from genital swabs. As a result, T. equigenitalis was detected in 12 mares and 1 stallion by PCR, although the bacteria were isolated from only 2 of the PCR-positive mares. CEM-infected and carrier horses were treated by a combination of chemotherapy and surgery. Subsequent follow-up testing over a 3-year period did not detect T. equigenitalis. It was demonstrated that PCR testing was more sensitive than isolation as a method for the detection of T. equigenitalis from genital swabs of horses in the field. It was therefore suggested that a combination of PCR testing and treatment were useful measures in the eradication of CEM from Japan.
评估了聚合酶链反应（PCR）作为根除马传染性子宫炎（CEM）现场应用检测方法的有效性。1998年至2001年期间，从日本4026匹纯种母马和种马中采集了7534份生殖道拭子，用于检测“高危”马匹以及进行常规监测检测。对原始标本和培养标本进行细菌分离以及PCR检测，以从生殖道拭子中检测马泰勒氏菌。结果，通过PCR在12匹母马和1匹种马中检测到马泰勒氏菌，尽管仅从2匹PCR阳性母马中分离出了该细菌。对感染CEM和携带病菌的马匹采用化疗和手术相结合的方法进行治疗。随后在3年期间的后续检测未检测到马泰勒氏菌。结果表明，作为从现场马匹生殖道拭子中检测马泰勒氏菌的方法，PCR检测比细菌分离更敏感。因此，建议将PCR检测和治疗相结合是从日本根除CEM的有效措施。
