Duquesne F, Pronost S, Laugier C, Petry S
AFSSA, Laboratoire d'Etudes et de Recherches en Pathologie Equine, IPC, Goustranville, 14430 Dozulé, France.
Res Vet Sci. 2007 Feb;82(1):47-9. doi: 10.1016/j.rvsc.2006.05.001. Epub 2006 Jun 27.
A direct-PCR assay was developed for the rapid detection of Taylorella equigenitalis, a Gram-negative bacterium responsible for contagious equine metritis (CEM) in Equidae. The bacteria may be detected in equine genital swabs without need for a preliminary step of DNA extraction or bacterial isolation. Specificity was determined with 125 isolates of T. equigenitalis, 24 isolates of Taylorella asinigenitalis, five commensal bacteria of the genital tract and a facultative intracellular pathogen of foals found in large concentration in soil. Our PCR is specific and amplified a 413-bp 16S ribosomal DNA product only in all T. equigenitalis.
开发了一种直接PCR检测方法，用于快速检测马泰勒菌，这是一种革兰氏阴性细菌，可导致马科动物传染性马子宫炎（CEM）。无需进行DNA提取或细菌分离的初步步骤，即可在马生殖器拭子中检测到该细菌。用125株马泰勒菌、24株驴泰勒菌、5种生殖道共生细菌以及在土壤中大量存在的马驹兼性细胞内病原体确定了特异性。我们的PCR具有特异性，仅在所有马泰勒菌中扩增出一个413bp的16S核糖体DNA产物。
