Ikura Yoko, Kudo Toshiaki
Institute of Physical and Chemical Research ( RIKEN ), Wako 351-0198, Japan.
J Gen Appl Microbiol. 1999 Oct;45(5):247-251. doi: 10.2323/jgam.45.247.
The isolation of poly-(L-lactide) (PLA)-degrading microorganisms was investigated. A PLA-degrading actinomycete, strain No. 3118, was isolated and tentatively identified as a member of the genus Amycolatopsis. The optimum conditions for degradation of PLA were 43 degrees C at about pH 7 in a mineral salt medium with a low concentration of organic nutrients (0.002% yeast extract). The original shape of PLA film (Mw=2.3x10(5) after sterilization, 20 &mgr;m thick) disappeared within 2 weeks. Lactic acid was detected after the film was incubated with culture supernatant.
对聚（L-丙交酯）（PLA）降解微生物的分离进行了研究。分离出了一株PLA降解放线菌，编号为3118，初步鉴定为拟无枝酸菌属的一员。在含有低浓度有机营养物（0.002%酵母提取物）的矿物盐培养基中，PLA降解的最佳条件是43℃、pH约为7。灭菌后Mw = 2.3×10⁵、厚度为20μm的PLA薄膜的原始形状在2周内消失。薄膜与培养上清液孵育后检测到了乳酸。
