Mittag T, Kornfeld P, Tormay A, Woo C
N Engl J Med. 1976 Mar 25;294(13):691-4. doi: 10.1056/NEJM197603252941303.
Since the blood and thymus of patients with myasthenia gravis may contain inhibitors of neuromuscular transmission that affect acetylcholine receptors of striated muscle, we used denervated rat muscle to test for inhibitors in 43 serums and 18 thymus glands from such patients. Seven per cent of serums inhibited the binding of 125l alpha-bungarotoxin to triton-solubilized receptors; 65 per cent interfered with binding of toxin-labeled receptors to concanavalin-A coupled to Sepharose gel, and 85 per cent formed IgG-receptor complexes detectable by immunoprecipitation. Serum inhibitory activity varied widely among patients with similar clinical manifestations and was not correlated with duration of myasthenia gravis or thymectomy. Among thymus extracts, 44 per cent were inhibitory in the concanavalin-A binding assay, whereas 72 per cent contained anti-receptor IgG. Thus, serums from patients with myasthenia gravis contain more than one anti-receptor factor.
由于重症肌无力患者的血液和胸腺可能含有影响横纹肌乙酰胆碱受体的神经肌肉传递抑制剂，我们使用去神经大鼠肌肉对43例此类患者的血清和18个胸腺进行抑制剂检测。7%的血清抑制125Iα-银环蛇毒素与经曲拉通溶解的受体的结合；65%干扰毒素标记的受体与偶联到琼脂糖凝胶上的伴刀豆球蛋白A的结合，85%形成可通过免疫沉淀检测到的IgG-受体复合物。血清抑制活性在临床表现相似的患者中差异很大，且与重症肌无力的病程或胸腺切除术无关。在胸腺提取物中，44%在伴刀豆球蛋白A结合试验中具有抑制作用，而72%含有抗受体IgG。因此，重症肌无力患者的血清含有不止一种抗受体因子。
