Nakano Akihiko
Molecular Membrane Biology Laboratory, RIKEN Discovery Research Institute, Saitama, Japan.
Cell Struct Funct. 2002 Oct;27(5):349-55. doi: 10.1247/csf.27.349.
Confocal laser scanning microscopy is experiencing a revolution in speed from the world of seconds to that of milliseconds. The spinning Nipkow disk method with microlenses has made this remarkable innovation possible. In combination with the ultrahigh-sensitivity, high-speed and high-resolution camera system based on avalanche multiplication of photoconduction, we are now able to observe the extremely dynamic movement of small vesicles in living cells in real time.
共聚焦激光扫描显微镜正经历一场速度革命，从以秒为单位的世界进入到以毫秒为单位的世界。带有微透镜的旋转尼普科夫盘方法使这一卓越创新成为可能。结合基于光导雪崩倍增的超高灵敏度、高速和高分辨率相机系统，我们现在能够实时观察活细胞中小囊泡极其动态的运动。
