Oreopoulos John, Berman Richard, Browne Mark
Spectral Applied Research, Richmond Hill, Ontario, Canada.
Andor Technology, Belfast, United Kingdom.
Methods Cell Biol. 2014;123:153-75. doi: 10.1016/B978-0-12-420138-5.00009-4.
Live-cell imaging requires not only high temporal resolution but also illumination powers low enough to minimize photodamage. Traditional single-point laser scanning confocal microscopy (LSCM) is generally limited by both the relatively slow speed at which it can acquire optical sections by serial raster scanning (a few Hz) and the higher potential for phototoxicity. These limitations have driven the development of rapid, parallel forms of confocal microscopy, the most popular of which is the spinning-disk confocal microscope (SDCM). Here, we briefly introduce the SDCM technique, discuss its strengths and weaknesses against LSCM, and update the reader on some recent developments in SDCM technology that improve its performance and expand its utility for life science research now and in the future.
活细胞成像不仅需要高时间分辨率，还需要足够低的照明功率以将光损伤降至最低。传统的单点激光扫描共聚焦显微镜（LSCM）通常受到两个限制，一是通过串行光栅扫描获取光学切片的速度相对较慢（几赫兹），二是具有更高的光毒性可能性。这些限制推动了快速、并行形式的共聚焦显微镜的发展，其中最流行的是转盘共聚焦显微镜（SDCM）。在这里，我们简要介绍SDCM技术，讨论其与LSCM相比的优缺点，并向读者介绍SDCM技术的一些最新进展，这些进展提高了其性能并扩展了其在当前和未来生命科学研究中的应用。
