Ellrott Kyle P, Kasarjian Julie K A, Jiang Tao, Ryu Junichi
University of California, Riverside, Riverside, CA, USA.
Biotechniques. 2002 Dec;33(6):1322-6. doi: 10.2144/02336bc02.
A critical and difficult part of characterizing restriction enzymes and methylases is the identification of recognition sequences. To simplify this process, we have developed a plasmid transformation method along with a computer program named RM search that determines the exact recognition sequences for given restriction and modification systems.
鉴定限制性内切酶和甲基化酶的一个关键且困难的部分是识别序列的确定。为了简化这一过程,我们开发了一种质粒转化方法以及一个名为RM search的计算机程序,该程序可确定给定限制和修饰系统的确切识别序列。