在大肠杆菌临床分离株中鉴定出四种新型I型限制酶。

Four new type I restriction enzymes identified in Escherichia coli clinical isolates.

作者信息

Kasarjian Julie K A, Kodama Yoshiaki, Iida Masatake, Matsuda Katsura, Ryu Junichi

机构信息

Division of Microbiology and Molecular Genetics, Department of Biochemistry and Microbiology, Loma Linda University, Loma Linda, CA 92350, USA.

出版信息

Nucleic Acids Res. 2005 Jul 21;33(13):e114. doi: 10.1093/nar/gni114.

DOI:10.1093/nar/gni114

PMID:16040596

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1178010/

Abstract

Using a plasmid transformation method and the RM search computer program, four type I restriction enzymes with new recognition sites and two isoschizomers (EcoBI and Eco377I) were identified in a collection of clinical Escherichia coli isolates. These new enzymes were designated Eco394I, Eco826I, Eco851I and Eco912I. Their recognition sequences were determined to be GAC(5N)RTAAY, GCA(6N)CTGA, GTCA(6N)TGAY and CAC(5N)TGGC, respectively. A methylation sensitivity assay, using various synthetic oligonucleotides, was used to identify the adenines that prevent cleavage when methylated (underlined). These results suggest that type I enzymes are abundant in E.coli and many other bacteria, as has been inferred from bacterial genome sequencing projects.

摘要

采用质粒转化方法和RM搜索计算机程序，在一组临床大肠杆菌分离株中鉴定出了四种具有新识别位点的I型限制酶以及两种同裂酶（EcoBI和Eco377I）。这些新酶被命名为Eco394I、Eco826I、Eco851I和Eco912I。它们的识别序列分别确定为GAC(5N)RTAAY、GCA(6N)CTGA、GTCA(6N)TGAY和CAC(5N)TGGC。使用各种合成寡核苷酸进行甲基化敏感性分析，以鉴定甲基化时阻止切割的腺嘌呤（下划线）。这些结果表明，正如从细菌基因组测序项目中推断的那样，I型酶在大肠杆菌和许多其他细菌中很丰富。