Chiuchetta S J R, Castro-Prado M A A
Department of Cell Biology and Genetics, State University of Maringá, 87 020-900 Maringá, Brazil.
Folia Microbiol (Praha). 2002;47(5):516-20. doi: 10.1007/BF02818791.
Recombinagenic potential of the alkaloid cryptolepine was evaluated in two diploid strains of Aspergillus nidulans--a wild type strain (uvsH+//uvsH+) and a DNA-repair-deficient one (uvsH//uvsH). Treatment of both strains with cryptolepine failed to alter colony growth in culture; its recombinagenic potential was determined by the homozygotization index (in which events of mitotic exchange may cause expression of genes previously masked by the dominant allele). Mitotic crossing-overs were induced by 7 and 14 mg/L doses of cryptolepine in both diploid A. nidulans strains.
在构巢曲霉的两个二倍体菌株中评估了生物碱隐丹参酮的重组潜能——一个野生型菌株（uvsH+//uvsH+）和一个DNA修复缺陷型菌株（uvsH//uvsH）。用隐丹参酮处理这两个菌株均未改变培养物中的菌落生长；其重组潜能由纯合化指数确定（在该指数中，有丝分裂交换事件可能导致先前被显性等位基因掩盖的基因表达）。在两个二倍体构巢曲霉菌株中，7和14 mg/L剂量的隐丹参酮诱导了有丝分裂交换。
