Osman F, Cotton C, Tomsett B, Strike P
Department of Genetics and Microbiology, University of Liverpool, UK.
Biochimie. 1991 Feb-Mar;73(2-3):321-7. doi: 10.1016/0300-9084(91)90219-q.
A mutant of Aspergillus nidulans, designated nuv11, has been isolated as hypersensitive to the monofunctional alkylating agent MNNG and the quasi-UV-mimetic mutagen 4-NQO. The mutation was recessive, resulting from mutation of a single gene which mapped to chromosome IV, and was non-allelic to the previously characterised repair-deficient mutations uvsB and uvsH which are also located on this linkage group. The nuv11 mutation results in slow growth, deficient intragenic and intergenic meiotic recombination, increased spontaneous chromosome instability, and increased intragenic and intergenic mitotic recombination in homozygous diploids. By screening a wild-type gene bank of A nidulans, a clone (pNUV11A40) has been isolated which complements the nuv11 mutation, restoring wild-type responses to both MNNG and 4-NQO.
已分离出一种构巢曲霉突变体，命名为nuv11，它对单功能烷化剂MNNG和类紫外线模拟诱变剂4-NQO高度敏感。该突变是隐性的，由一个单基因突变引起，该基因定位于第四条染色体，并且与先前表征的位于同一连锁群上的修复缺陷突变uvsB和uvsH是非等位基因。nuv11突变导致生长缓慢、基因内和基因间减数分裂重组缺陷、自发染色体不稳定性增加，以及纯合二倍体中基因内和基因间有丝分裂重组增加。通过筛选构巢曲霉的野生型基因文库，分离出一个克隆（pNUV11A40），它能互补nuv11突变，恢复对MNNG和4-NQO的野生型反应。
