酵母中ash1p的不对称分选源于mRNA中定位元件对翻译的抑制作用。
Asymmetric sorting of ash1p in yeast results from inhibition of translation by localization elements in the mRNA.
作者信息
Chartrand Pascal, Meng Xiu Hua, Huttelmaier Stefan, Donato Damiane, Singer Robert H
机构信息
Department of Anatomy and Structural Biology, Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
出版信息
Mol Cell. 2002 Dec;10(6):1319-30. doi: 10.1016/s1097-2765(02)00694-9.
ASH1 mRNA localizes at the bud tip of late-anaphase yeast, resulting in accumulation of Ash1p in the daughter nucleus. We show that disruption of the secondary structure, but not the protein coding, of all four ASH1 localization elements resulted in RNA and protein delocalization. Localization of both was incrementally restored by replacement of each of the four elements. However, transposition of the elements to the 3'UTR reinstated the RNA, but not the protein, localization. Interestingly, the mutant ASH1 mRNA was translated more efficiently, suggesting that asymmetry of Ash1p resulted from translational inhibition by the localization elements. In support of this, Ash1p asymmetry could be rescued by slowing its translation.
ASH1信使核糖核酸定位于酵母细胞后期的芽尖，导致Ash1蛋白在子细胞核中积累。我们发现，所有四个ASH1定位元件的二级结构被破坏，但蛋白质编码未受影响，会导致核糖核酸和蛋白质的定位紊乱。通过替换四个元件中的每一个，两者的定位逐渐恢复。然而，将这些元件转位到3'非翻译区可恢复核糖核酸的定位，但不能恢复蛋白质的定位。有趣的是，突变的ASH1信使核糖核酸翻译效率更高，这表明Ash1蛋白的不对称性是由定位元件的翻译抑制导致的。支持这一观点的是，减缓Ash1蛋白的翻译可以挽救其不对称性。
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