使用偶极离子交换剂对转移核糖核酸进行分级分离。
The use of a dipolar ion-exchanger for the fractionation of transfer ribonucleic acid.
作者信息
Jay F T, Coultas C, Jones D S
出版信息
Nucleic Acids Res. 1976 Jan;3(1):177-90. doi: 10.1093/nar/3.1.177.
Transfer ribonucleic acid is well fractionated on columns of arginine-agarose, whose properties appear in general to be similar to those of DEAE-Sephadex. However, the amino acid acceptor species are separated into sharper peaks and in several instances, notably for methionine, glycine, serine, leucine and aspartate accepting tRNAs from Escherichia coli, isoaccepting species are well resolved. In the case of methionine accepting tRNA from E. coli the tRNA Met-m species is eluted before the tRNA Met-f species and since it is also eluted prior to the bulk of the tRNA it is obtained in a high degree of purity. By comparing the properties of columns of arginine-agarose and its methyl ester in which the carboxylate anion is blocked, it is seen that the carboxylate ion plays a role in the fractionation of the tRNA Met species.
转移核糖核酸在精氨酸 - 琼脂糖柱上能得到很好的分离，其性质总体上似乎与二乙氨基乙基 - 葡聚糖凝胶（DEAE - Sephadex）相似。然而，氨基酸接受种类被分离成更尖锐的峰，并且在几种情况下，特别是对于来自大肠杆菌的甲硫氨酸、甘氨酸、丝氨酸、亮氨酸和天冬氨酸接受tRNA，同功受体种类得到了很好的分离。就来自大肠杆菌的甲硫氨酸接受tRNA而言，tRNA Met - m种类在tRNA Met - f种类之前被洗脱，并且由于它也在大部分tRNA之前被洗脱，所以它能以高纯度获得。通过比较精氨酸 - 琼脂糖柱及其甲酯（其中羧酸根阴离子被封闭）的性质，可以看出羧酸根离子在tRNA Met种类的分馏中起作用。
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