Nocianitri Komang Ayu, Sakakibara Shoji, Kanno Takashi, Kikuchi Hiroto, Kurasaki Masaaki, Aoyama Yoritaka
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Nishi-9, Kita-9, Kita-ku, Sapporo 060-8589, Japan.
Biosci Biotechnol Biochem. 2002 Nov;66(11):2465-70. doi: 10.1271/bbb.66.2465.
The effects of some methyl-containing compounds added to a choline-deficient diet on the metallothionein mRNA level in the rat liver were studied. The addition of choline or carnitine to the choline-deficient diet did not induce a gain in body weight, while the addition of either betaine or methionine to the choline-deficient diet, or of methionine to the choline-deficient diet with choline significantly increased the body weight. The metallothionein mRNA level in the liver of rats fed on the choline-deficient diet was similar to that of rats fed on the choline-deficient diet with choline, betaine or carnitine. However, the addition of methionine to the choline-deficient diet with or without choline caused a marked suppression in the metallothionein mRNA level in the liver. It is thus surmised that the metallothionein mRNA level in the liver might be regulated by the dietary content of methionine.
研究了在胆碱缺乏饮食中添加某些含甲基化合物对大鼠肝脏金属硫蛋白mRNA水平的影响。在胆碱缺乏饮食中添加胆碱或肉碱不会使体重增加，而在胆碱缺乏饮食中添加甜菜碱或蛋氨酸，或在胆碱缺乏饮食中添加胆碱的同时添加蛋氨酸，均能显著增加体重。饲喂胆碱缺乏饮食的大鼠肝脏中金属硫蛋白mRNA水平与饲喂含胆碱、甜菜碱或肉碱的胆碱缺乏饮食的大鼠相似。然而，在含或不含胆碱的胆碱缺乏饮食中添加蛋氨酸会导致肝脏中金属硫蛋白mRNA水平显著降低。因此推测，肝脏中金属硫蛋白mRNA水平可能受饮食中蛋氨酸含量的调节。
