Dean M F, Muir H
Biochem J. 1975 Mar;146(3):557-63. doi: 10.1042/bj1460557.
Proteoglycan from Kurloff cells, when present in the medium in low concentrations, increased the incorporation of (3-H)leucine into trichloracetic acid-precipitable material by macrophages from peritoneal exudates, in addition to inhibiting their migration from capillary tubes, as observed previously. After treatment with 0.5 M-NaOH, followed by dialysis or ultrafiltration, material with the distinctive u.v. and i.r. spectra of the whole proteoglycan appeared in the diffusate, and biological activity was lost from the proteoglycan which remained in the dialysis residue. The diffusible material absorbed near 260 nm and had i.r. bands at 805 cm-minus-1 and 1260 cm-minus-1, but did not display the i.r. bands characteristic of chondrotin 4-sulphate. It contained little sulphate, no hexosamine and less than 1% of the uronic acid present in the whole proteoglycan, and there were only trace amounts of amino acids, xylose and galactose. However, significant amounts of ribose and organic phosphate were present, each representing about 1% of the whole proteoglycan. After proteolysis and chondroitanase digestion of the proteoglycan, a fraction with absorbance at 260 mn was eluted from Dowex 1 with water which stimulated the incorporation of (3H)leucine by macrophages and inhibited their migration from capillary tubes.
库尔洛夫细胞中的蛋白聚糖，当以低浓度存在于培养基中时，除了如先前观察到的那样抑制巨噬细胞从毛细管迁移外，还增加了来自腹膜渗出液的巨噬细胞将（3-H）亮氨酸掺入三氯乙酸可沉淀物质中的量。用0.5M氢氧化钠处理，然后进行透析或超滤后，具有整个蛋白聚糖独特紫外和红外光谱的物质出现在扩散液中，而保留在透析残渣中的蛋白聚糖失去了生物活性。可扩散物质在260nm附近有吸收，在805cm-1和1260cm-1处有红外波段，但没有显示出软骨素4-硫酸盐的特征红外波段。它含有少量硫酸盐，没有己糖胺，整个蛋白聚糖中存在的糖醛酸含量不到1%，并且只有微量的氨基酸、木糖和半乳糖。然而，存在大量的核糖和有机磷酸盐，每种约占整个蛋白聚糖的1%。对蛋白聚糖进行蛋白水解和软骨素酶消化后，用水从Dowex 1上洗脱了在260nm处有吸光度的部分，该部分刺激巨噬细胞掺入（3H）亮氨酸并抑制其从毛细管迁移。
