PTEN编码产物：胃癌发生和进展的标志物
PTEN encoding product: a marker for tumorigenesis and progression of gastric carcinoma.
作者信息
Yang Lin, Kuang Li-Ge, Zheng Hua-Chuan, Li Jin-Yi, Wu Dong-Ying, Zhang Su-Min, Xin Yan
机构信息
No.4 Lab, Cancer Institute, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
出版信息
World J Gastroenterol. 2003 Jan;9(1):35-9. doi: 10.3748/wjg.v9.i1.35.
AIM
To detect the expression of PTEN encoding product in normal mucosa, intestinal metaplasia (IM), dysplasia and carcinoma of the stomach, and to investigate its clinical implication in tumorigenesis and progression of gastric carcinoma.
METHODS
Formalin-fixed paraffin embedded specimens from 184 cases of gastric carcinoma, their adjacent normal mucosa, IM and dysplasia were evaluated for PTEN protein expression by SABC immunohistochemistry. PTEN expression was compared with tumor stage, lymph node metastasis, Lauren's and WHO's histological classification of gastric carcinoma. Expression of VEGF was also detected in 60 cases of gastric carcinoma and its correlation with PTEN was concerned.
RESULTS
The positive rates of PTEN protein were 100 %(102/102), 98.5 %(65/66), 66.7 % (4/6) and 47.8 %(88/184) in normal mucosa, IM, dysplasia and carcinoma of the stomach, respectively. The positive rates in dysplasia and carcinoma were lower than in normal mucosa and IM (P<0.01). Advanced gastric cancers expressed less frequent PTEN than early gastric cancer (42.9 % vs 67.6 %, P<0.01). The positive rate of PTEN protein was lower in gastric cancer with than without lymph node metastasis (40.3 % vs 63.3 %, P<0.01). PTEN was less expressed in diffuse-type than in intestinal-type gastric cancer (41.5 % vs 57.8 %, P<0.05). Signet ring cell carcinoma showed the expression of PTEN at the lowest level (25.0 %, 7/28); less than well and moderately differentiated ones (P<0.01). Expression of PTEN was not correlated with expression of VEGF (P>0.05).
CONCLUSION
Loss or reduced expression of PTEN protein occures commonly in tumorigenesis and progression of gastric carcinoma. It is suggested that PTEN can be an objective marker for pathologically biological behaviors of gastric carcinoma.
目的
检测PTEN编码产物在胃正常黏膜、肠化生（IM）、发育异常及癌组织中的表达，探讨其在胃癌发生、发展中的临床意义。
方法
采用SABC免疫组化法检测184例胃癌及其癌旁正常黏膜、IM和发育异常组织中PTEN蛋白的表达。将PTEN表达与肿瘤分期、淋巴结转移、Lauren分型及WHO胃癌组织学分类进行比较。同时检测60例胃癌组织中VEGF的表达，并分析其与PTEN的相关性。
结果
PTEN蛋白在胃正常黏膜、IM、发育异常及癌组织中的阳性率分别为100%（102/102）、98.5%（65/66）、66.7%（4/6）和47.8%（88/184）。发育异常及癌组织中的阳性率低于正常黏膜及IM（P<0.01）。进展期胃癌PTEN表达低于早期胃癌（42.9%对67.6%，P<0.01）。有淋巴结转移的胃癌PTEN蛋白阳性率低于无淋巴结转移者（40.3%对63.3%，P<0.01）。弥漫型胃癌PTEN表达低于肠型胃癌（41.5%对57.8%，P<0.05）。印戒细胞癌PTEN表达最低（25.0%，7/28），低于高分化及中分化癌（P<0.01）。PTEN表达与VEGF表达无相关性（P>0.05）。
结论
PTEN蛋白在胃癌发生、发展过程中常见缺失或表达降低。提示PTEN可作为判断胃癌病理生物学行为的客观指标。
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