Zheng Hua-chuan, Chen Ying, Kuang Li-ge, Yang Lin, Li Jin-yi, Wu Dong-ying, Zhang Su-min, Xin Yan
Fourth Laboratory, Cancer Institute, First Hospital, China Medical University, Shenyang 110001, China.
Zhonghua Zhong Liu Za Zhi. 2003 Jan;25(1):13-6.
To illustrate the significance of expression of phosphatase and tensin homologue derived from chromosome ten (PTEN) encoding product in normal mucosa, intestinal metaplasia (IM), dysplasia and carcinoma of the stomach, and to evaluate its clinical implication in tumorigenesis and progression of gastric carcinoma.
Formalin-fixed and paraffin-embedded tissues from 184 cases of gastric carcinoma, its adjacent normal mucosa, IM and dysplasia were evaluated for the expression of PTEN by SABC immunohistochemistry. PTEN expression was assessed as to tumor stage, lymph node metastasis, Lauren's classification and WHO histological classification of gastric carcinoma. Expression of VEGF protein was also studied in 60 cases of gastric carcinoma, with its correlation with PTEN concerned.
The positive rates of PTEN protein were 100% (102/102), 98.5% (65/66), 66.7% (4/6) and 47.8% (88/184) in normal mucosa, IM, dysplasia and carcinoma of stomach, respectively. The positive rates in the last two groups were lower than the first two (P < 0.01). PTEN was less expressed in advanced gastric carcinoma than in early ones (42.9% vs 67.6%, P < 0.01). The positive rate of PTEN protein was lower in gastric carcinoma with lymph node metastasis than without (40.3% vs 63.3%, P < 0.01). PTEN was less expressed in diffuse-type gastric carcinoma than in intestinal-type (41.5% vs 57.8%, P < 0.05). Signet ring cell carcinoma expressed PTEN stood the lowest (25.0%, 7/28), which was less than well and moderately differentiated ones (61.8%, 21/34) (P < 0.01). Expression of PTEN was inversely correlated with expression of VEGF though without any significance (P > 0.05).
Loss or reduced expression of PTEN protein is common in carcinogenesis and progression of gastric cancer. Altered expression of PTEN may contribute to carcinogenesis and progression of gastric cancer by increasing angiogenesis, cellular adhesion and mobility and so on. PTEN may be an objective marker for pathologically biological behavior of gastric carcinoma.
阐明10号染色体缺失的磷酸酶和张力蛋白同源物（PTEN）编码产物在胃正常黏膜、肠化生（IM）、发育异常及癌组织中的表达意义，并评估其在胃癌发生及进展中的临床意义。
采用SABC免疫组化法检测184例胃癌及其癌旁正常黏膜、IM和发育异常组织中PTEN的表达情况。对PTEN表达与肿瘤分期、淋巴结转移、Lauren分型及WHO胃癌组织学分类进行评估。同时研究60例胃癌组织中VEGF蛋白表达情况，并分析其与PTEN的相关性。
PTEN蛋白在胃正常黏膜、IM、发育异常及癌组织中的阳性率分别为100%（102/102）、98.5%（65/66）、66.7%（4/6）和47.8%（88/184）。后两组阳性率低于前两组（P<0.01）。进展期胃癌PTEN表达低于早期胃癌（42.9%对67.6%，P<0.01）。有淋巴结转移的胃癌PTEN蛋白阳性率低于无淋巴结转移者（40.3%对63.3%，P<0.01）。弥漫型胃癌PTEN表达低于肠型胃癌（41.5%对57.8%，P<0.05）。印戒细胞癌PTEN表达最低（25.0%，7/28），低于高、中分化腺癌（61.8%，21/34）（P<0.01）。PTEN表达与VEGF表达呈负相关，但差异无统计学意义（P>0.05）。
PTEN蛋白表达缺失或降低在胃癌发生及进展过程中常见。PTEN表达改变可能通过增加血管生成、细胞黏附及迁移等促进胃癌的发生及进展。PTEN可能是胃癌病理生物学行为的客观标志物。
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