Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Can J Gastroenterol Hepatol. 2022 Aug 25;2022:8699408. doi: 10.1155/2022/8699408. eCollection 2022.
and genes contribute to the cell cycle during proliferation and apoptosis, respectively. Designing a case-control study including gastric adenocarcinoma and gastritis patients with and without infection would lead to determinate of the correlations between and genes expression with infection in gastric antral epithelial cells.
Overall, 50 and 30 gastric antral biopsy samples of gastric cancer (case group) and gastritis (control group) patients were included into study, respectively. All biopsy samples were collected considering the exclusion criteria including patients with a history of consumption of tobacco, alcohol, and anti- drugs. Each patient group is divided into with and without infection to detect cDNA fold changes of and genes by using Real Time RT PCR. Furthermore, the presence of virulence genes was detected directly by using specific primers and simple PCR on cDNA synthesized from total RNA of gastric antral biopsy samples.
A negative correlation was revealed between age and clinical manifestations with the ΔCt value of the gene ( < 0.05). The and genes revealed positive and negative correlations with the ΔCt value of the gene ( < 0.05), respectively. Furthermore, a weak correlation was detectable between , and genes and the ΔCt value of the gene in gastric antral epithelial cells of patients ( < 0.1).
The results of the current study opened a view for more investigation on the stunning roles of infection in clinical outcomes through and gene expression in gastric antral epithelial cells.
和基因分别在增殖和细胞凋亡过程中参与细胞周期。设计一个包括胃腺癌和胃炎患者的病例对照研究，有和没有感染，将有助于确定胃窦上皮细胞中与感染相关的和基因表达之间的相关性。
总体上，包括 50 例胃癌（病例组）和 30 例胃炎（对照组）患者的胃窦活检样本被纳入研究。所有活检样本均采集，考虑到排除标准包括患者有吸烟、饮酒和抗酸药物史。每个患者组分为有和没有感染，通过实时 RT-PCR 检测和基因的 cDNA 折叠变化。此外，通过使用特异性引物和简单 PCR 直接检测从胃窦活检样本总 RNA 合成的 cDNA 上的毒力基因的存在。
年龄和临床表现与基因的ΔCt 值呈负相关（<0.05）。和基因与基因的ΔCt 值呈正相关和负相关（<0.05）。此外，在患者的胃窦上皮细胞中，基因和基因之间可以检测到较弱的相关性（<0.1）。
本研究的结果为进一步研究感染通过和基因在胃窦上皮细胞中的表达对临床结局的惊人作用开辟了视野。
