and Genes Expression in Gastritis and Gastric Cancer Patients with Infection.

BACKGROUND

and genes contribute to the cell cycle during proliferation and apoptosis, respectively. Designing a case-control study including gastric adenocarcinoma and gastritis patients with and without infection would lead to determinate of the correlations between and genes expression with infection in gastric antral epithelial cells.

METHODS

Overall, 50 and 30 gastric antral biopsy samples of gastric cancer (case group) and gastritis (control group) patients were included into study, respectively. All biopsy samples were collected considering the exclusion criteria including patients with a history of consumption of tobacco, alcohol, and anti- drugs. Each patient group is divided into with and without infection to detect cDNA fold changes of and genes by using Real Time RT PCR. Furthermore, the presence of virulence genes was detected directly by using specific primers and simple PCR on cDNA synthesized from total RNA of gastric antral biopsy samples.

RESULTS

A negative correlation was revealed between age and clinical manifestations with the ΔCt value of the gene ( < 0.05). The and genes revealed positive and negative correlations with the ΔCt value of the gene ( < 0.05), respectively. Furthermore, a weak correlation was detectable between , and genes and the ΔCt value of the gene in gastric antral epithelial cells of patients ( < 0.1).

CONCLUSIONS

The results of the current study opened a view for more investigation on the stunning roles of infection in clinical outcomes through and gene expression in gastric antral epithelial cells.