Xiong Li-Juan, Zhu Jian-Fang, Luo Duan-De, Zen Lin-Lan, Cai Shu-Qing
Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
World J Gastroenterol. 2003 Jan;9(1):152-4. doi: 10.3748/wjg.v9.i1.152.
To study the effects of pentoxifylline (PTX) on the content of hepatic TGF-beta1, type I and type III collagen in schistosomiasis japonica mice with liver fibrosis and its mechanism of anti-fibrosis.
Forty mice with schistosomiasis were divided into four groups: one group as control without any treatment, other three were treated with Praziquantel 500 mg/(kg x d)for 2 d, high dose PTX 360 mg/(kg x d) for 8 wk, and low dose PTX 180 mg/(kg x d) for 8 wk respectively. Immunohistochemical technique and multimedia color pathographic analysis system were applied to observe the content change of hepatic TGF-beta1, type I and type III collagen in schistosomiasis japonica mice with liver fibrosis before and after PTX treatment.
Effects of PTX on the content change of hepatic TGF-beta1, type I and type III collagen in schistosomiasis japonica mice with liver fibrosis were related to the dosage of PTX, high dose PTX treated group could significantly reduce the content of TGF-beta1 (0.709+/-0.111), type I (0.644+/-0.108) and type III (0.654+/-0.152) collagen compared with those of control group (0.883+/-0.140, 0.771+/-0.156, 0.822+/-0.129) with statistical significance (P<0.05). Low dose PTX could also reduce the hepatic content of TGF-beta1 (0.752+/-0.152), type I (0.733+/-0.117) and type III (0.788+/-0.147) collagen, but without statistical significance (P>0.05). Both high dose and low dose PTX groups have significant differences on the content of TGF-beta1, type I and type III collagen (P<0.05, P<0.05, P<0.01, respectively).
High dose of PTX treatment could reduce the content of hepatic TGF-beta1, type I and type III collagen significantly in schistosomiasis japonica mice with liver fibrosis, and thus plays its role of antifibrosis.
研究己酮可可碱（PTX）对日本血吸虫病肝纤维化小鼠肝脏转化生长因子-β1（TGF-β1）、Ⅰ型和Ⅲ型胶原蛋白含量的影响及其抗纤维化机制。
将40只日本血吸虫病小鼠分为四组：一组作为对照组不做任何处理，其他三组分别用吡喹酮500mg/（kg·d）治疗2天、高剂量PTX 360mg/（kg·d）治疗8周、低剂量PTX 180mg/（kg·d）治疗8周。应用免疫组织化学技术和多媒体彩色病理图像分析系统观察PTX治疗前后日本血吸虫病肝纤维化小鼠肝脏TGF-β1、Ⅰ型和Ⅲ型胶原蛋白含量变化。
PTX对日本血吸虫病肝纤维化小鼠肝脏TGF-β1、Ⅰ型和Ⅲ型胶原蛋白含量变化的影响与PTX剂量有关，高剂量PTX治疗组与对照组相比，可显著降低TGF-β1（0.709±0.111）、Ⅰ型（0.644±0.108）和Ⅲ型（0.654±0.152）胶原蛋白含量（对照组分别为0.883±0.140、0.771±0.156、0.822±0.129），差异有统计学意义（P<0.05）。低剂量PTX也可降低肝脏TGF-β1（0.752±0.152）、Ⅰ型（0.733±0.117）和Ⅲ型（0.788±0.147）胶原蛋白含量，但差异无统计学意义（P>0.05）。高剂量和低剂量PTX组在TGF-β1、Ⅰ型和Ⅲ型胶原蛋白含量上均有显著差异（分别为P<0.05、P<0.05、P<0.01）。
高剂量PTX治疗可显著降低日本血吸虫病肝纤维化小鼠肝脏TGF-β1、Ⅰ型和Ⅲ型胶原蛋白含量，从而发挥其抗纤维化作用。
