银杏叶提取物可逆转四氯化碳诱导的大鼠肝纤维化。
Ginkgo biloba extract reverses CCl4-induced liver fibrosis in rats.
作者信息
Luo Yan-Jun, Yu Jie-Ping, Shi Zhao-Hong, Wang Li
机构信息
Department of Gastroenterology, Hubei Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.
出版信息
World J Gastroenterol. 2004 Apr 1;10(7):1037-42. doi: 10.3748/wjg.v10.i7.1037.
AIM
To study the reversing effect of Ginkgo biloba extract (GbE) on established liver fibrosis in rats.
METHODS
Following confirmation of CCl(4)-induced liver fibrosis, GbE or saline was administrated to the rats for 4 weeks. The remaining rats received neither CCl(4) nor GbE as normal control. The four groups were compared in terms of serum enzymes, tissue damage, expression of alphaSMA and tissue inhibitor-1 of metalloproteinase (TIMP-1) and metalloproteinase-1 (MMP-1).
RESULTS
Compared with saline-treated group, liver fibrosis rats treated with GbE had decreased serum total bilirubin (P<0.01) and aminotransferase levels (P<0.01) and increased levels of serum albumin (P<0.01). Microscopic studies revealed that the livers of rats receiving GbE showed alleviation in fibrosis (P<0.05) as well as expression of alphaSMA (P<0.01). The liver collagen and reticulum contents were lower in rats treated with GbE than saline-treated group (P<0.01). RT-PCR revealed that the level of TIMP-1 decreased while the level of MMP-1 increased in GbE group.
CONCLUSION
Administration of GbE improved CCl(4)-induced liver fibrosis. It is possibly attributed to its effect of inhibiting the expression of TIMP-1 and promoting the apoptosis of hepatic stellate cells.
目的
研究银杏叶提取物（GbE）对大鼠已形成的肝纤维化的逆转作用。
方法
在确认四氯化碳诱导的肝纤维化后，给大鼠施用GbE或生理盐水，持续4周。其余大鼠既不接受四氯化碳也不接受GbE作为正常对照。比较四组大鼠的血清酶、组织损伤、α平滑肌肌动蛋白（alphaSMA）的表达以及金属蛋白酶组织抑制剂-1（TIMP-1）和金属蛋白酶-1（MMP-1）的水平。
结果
与生理盐水处理组相比，用GbE处理的肝纤维化大鼠血清总胆红素降低（P<0.01），转氨酶水平降低（P<0.01），血清白蛋白水平升高（P<0.01）。显微镜研究显示，接受GbE的大鼠肝脏纤维化减轻（P<0.05），alphaSMA表达降低（P<0.01）。用GbE处理的大鼠肝脏胶原蛋白和网状纤维含量低于生理盐水处理组（P<0.01）。逆转录聚合酶链反应（RT-PCR）显示，GbE组TIMP-1水平降低，而MMP-1水平升高。
结论
施用GbE可改善四氯化碳诱导的肝纤维化。这可能归因于其抑制TIMP-1表达和促进肝星状细胞凋亡的作用。
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