van Mastrigt Esther, Zweekhorst Salomé, Bol Bas, Tibboel Jeroen, van Rosmalen Joost, Samsom Janneke N, Kroon André A, de Jongste Johan C, Reiss Irwin K M, Post Martin, Pijnenburg Mariëlle W
Division of Pediatric Pulmonology, Erasmus MC-Sophia Children's Hospital, Rotterdam, the Netherlands.
Division of Neonatology, Erasmus MC-Sophia Children's Hospital, Rotterdam, the Netherlands.
PLoS One. 2018 Jan 18;13(1):e0185969. doi: 10.1371/journal.pone.0185969. eCollection 2018.
In an experimental mouse model we showed that ceramides play a role in the pathogenesis of bronchopulmonary dysplasia (BPD) and are a potential target for therapeutic intervention. We investigated whether ceramides are detectable in tracheal aspirates (TAs) of preterm infants and differ between infants with or without BPD.
Infants born ≤ 32 weeks of gestational age in need of mechanical ventilation in the first week of life were included. TAs were obtained directly after intubation and at day 1, 3, 5, 7, and 14. Ceramide concentrations were measured by tandem mass spectrometry. At 36 weeks postmenstrual age BPD was defined as having had ≥ 28 days supplemental oxygen.
122 infants were included, of which 14 died and 41 developed BPD. All infants showed an increase in ceramides after the first day of intubation. The ceramide profile differed significantly between preterm infants who did and did not develop BPD. However, the ceramide profile had no additional predictive value for BPD development over GA at birth, birth weight and total days of mechanical ventilation.
Ceramides are measurable in TAs of preterm born infants and may be an early marker for BPD development.
在一个实验性小鼠模型中，我们发现神经酰胺在支气管肺发育不良（BPD）的发病机制中起作用，并且是治疗干预的潜在靶点。我们研究了在早产儿的气管吸出物（TA）中是否可检测到神经酰胺，以及患BPD和未患BPD的婴儿之间神经酰胺是否存在差异。
纳入出生孕周≤32周、出生后第一周需要机械通气的婴儿。在插管后以及出生后第1、3、5、7和14天直接获取TA。通过串联质谱法测量神经酰胺浓度。在月经龄36周时，BPD定义为吸氧≥28天。
纳入122例婴儿，其中14例死亡，41例发生BPD。所有婴儿在插管后第一天后神经酰胺水平均升高。患BPD和未患BPD的早产儿的神经酰胺谱有显著差异。然而，对于BPD的发生，神经酰胺谱在出生孕周、出生体重和机械通气总天数之外没有额外的预测价值。
早产儿的TA中可测量到神经酰胺，并且可能是BPD发生的早期标志物。
