Zik Moriyah, Irish Vivian F
Department of Molecular, Cellular, and Developmental Biology, Osborn Memorial Laboratories, Yale University, New Haven, Connecticut 06520, USA.
Plant Cell. 2003 Jan;15(1):207-22. doi: 10.1105/tpc.006353.
Identifying the genes regulated by the floral homeotic genes APETALA3 (AP3) and PISTILLATA (PI) is crucial for understanding the molecular mechanisms that lead to petal and stamen formation. We have used microarray analysis to conduct a broad survey of genes whose expression is affected by AP3 and PI activity. DNA microarrays consisting of 9216 Arabidopsis ESTs were screened with probes corresponding to mRNAs from different mutant and transgenic lines that misexpress AP3 and/or PI. The microarray results were further confirmed by RNA gel blot analyses. Our results suggest that AP3 and PI regulate a relatively small number of genes, implying that many genes used in petal and stamen development are not tissue specific and likely have roles in other processes as well. We recovered genes similar to previously identified petal- and stamen-expressed genes as well as genes that were not implicated previously in petal and stamen development. A very low percentage of the genes recovered encoded transcription factors. This finding suggests that AP3 and PI act relatively directly to regulate the genes required for the basic cellular processes responsible for petal and stamen morphogenesis.
识别由花同源异型基因APETALA3(AP3)和PISTILLATA(PI)调控的基因,对于理解导致花瓣和雄蕊形成的分子机制至关重要。我们利用微阵列分析对其表达受AP3和PI活性影响的基因进行了广泛的调查。用与来自不同错表达AP3和/或PI的突变体和转基因系的mRNA相对应的探针,筛选了由9216个拟南芥EST组成的DNA微阵列。微阵列结果通过RNA凝胶印迹分析进一步得到证实。我们的结果表明,AP3和PI调控相对少量的基因,这意味着许多用于花瓣和雄蕊发育的基因不是组织特异性的,并且可能在其他过程中也发挥作用。我们回收了与先前鉴定的花瓣和雄蕊表达基因相似的基因,以及先前未涉及花瓣和雄蕊发育的基因。回收的基因中编码转录因子的比例非常低。这一发现表明,AP3和PI相对直接地作用于调控负责花瓣和雄蕊形态发生的基本细胞过程所需的基因。