Sablowski R W, Meyerowitz E M
California Institute of Technology, Division of Biology, Pasadena 91125, USA.
Cell. 1998 Jan 9;92(1):93-103. doi: 10.1016/s0092-8674(00)80902-2.
To understand how homeotic genes affect morphogenesis and differentiation, their target genes must be identified. In Arabidopsis flowers, the homeotic protein heterodimer APETALA3/PISTILLATA is necessary for petal and stamen formation. Here, AP3/PI function was put under posttranslational control to analyze its immediate effect on the floral mRNA population, with indirect effects blocked by cycloheximide. Using differential display, a target gene of AP3/PI was identified (NAP:NAC-LIKE, ACTIVATED BY AP3/PI), which is homologous to genes required for meristem establishment and separation of floral organs. The expression pattern of NAP and the phenotypes caused by its misexpression suggest that it functions in the transition between growth by cell division and cell expansion in stamens and petals.
为了了解同源异型基因如何影响形态发生和分化，必须鉴定它们的靶基因。在拟南芥花中，同源异型蛋白异二聚体APETALA3/PISTILLATA是花瓣和雄蕊形成所必需的。在这里，AP3/PI功能受到翻译后调控，以分析其对花mRNA群体的直接影响，同时用环己酰亚胺阻断间接影响。通过差异显示，鉴定出AP3/PI的一个靶基因（NAP：由AP3/PI激活的NAC样基因），它与分生组织建立和花器官分离所需的基因同源。NAP的表达模式及其错误表达所导致的表型表明，它在雄蕊和花瓣中细胞分裂生长与细胞扩张之间的转变过程中发挥作用。
