通过RNA诱导的基因沉默探索植物基因组。

Exploring plant genomes by RNA-induced gene silencing.

作者信息

Waterhouse Peter M, Helliwell Christopher A

机构信息

CSIRO Plant Industry, Canberra, Australian Capital Territory 2601, Australia.

出版信息

Nat Rev Genet. 2003 Jan;4(1):29-38. doi: 10.1038/nrg982.

DOI:10.1038/nrg982

PMID:12509751

Abstract

The nucleotide sequences of several animal, plant and bacterial genomes are now known, but the functions of many of the proteins that they are predicted to encode remain unclear. RNA interference is a gene-silencing technology that is being used successfully to investigate gene function in several organisms--for example, Caenorhabditis elegans. We discuss here that RNA-induced gene silencing approaches are also likely to be effective for investigating plant gene function in a high-throughput, genome-wide manner.

摘要

目前，几种动物、植物和细菌基因组的核苷酸序列已为人所知，但它们预计编码的许多蛋白质的功能仍不清楚。RNA干扰是一种基因沉默技术，已成功用于研究多种生物（如秀丽隐杆线虫）中的基因功能。我们在此讨论，RNA诱导的基因沉默方法也可能有效地以高通量、全基因组方式研究植物基因功能。