[cDNA array in the establishment of a gene expression profile associated with differentiation inducing the glioma cells].

OBJECTIVE

Establishment of a gene expression profile associated with differentiation inducing the glioma cells was made possible.

METHOD

The expression level of 18 000 genes in glioma cells was evaluated before and after induction with sodium phenyl-butyrate for 2 hours or 6 days by cDNA array technique, with the results proved by multi-dot blot.

RESULTS

Ninety-eight gene expressions in the glioma cells were changed after the induction, with some genes in transcription and translation systems down-regulated, some oncogenes down-regulated, and some differentiation or apoptosis genes up-regulated. Eighteen unknown EST fragments were changed also.

CONCLUSION

A gene expression profile associated with differentiation-inducing the glioma cells including 98 genes has been established.