Sun Lijun, Huang Qiang, Wang Aidong, Lan Qing, Du Ziwei, Hu Gengxi
Department of Neurosurgery, Second Hospital, Suzhou University, Suzhou 215004, China.
Zhonghua Zhong Liu Za Zhi. 2002 May;24(3):222-5.
Establishment of a gene expression profile associated with differentiation inducing the glioma cells was made possible.
The expression level of 18 000 genes in glioma cells was evaluated before and after induction with sodium phenyl-butyrate for 2 hours or 6 days by cDNA array technique, with the results proved by multi-dot blot.
Ninety-eight gene expressions in the glioma cells were changed after the induction, with some genes in transcription and translation systems down-regulated, some oncogenes down-regulated, and some differentiation or apoptosis genes up-regulated. Eighteen unknown EST fragments were changed also.
A gene expression profile associated with differentiation-inducing the glioma cells including 98 genes has been established.
实现与诱导胶质瘤细胞分化相关的基因表达谱的建立。
采用cDNA芯片技术评估苯丁酸钠诱导胶质瘤细胞2小时或6天后18000个基因的表达水平，并通过多点杂交进行结果验证。
诱导后胶质瘤细胞中有98个基因的表达发生改变，其中一些转录和翻译系统中的基因下调，一些癌基因下调，一些分化或凋亡基因上调。18个未知EST片段也发生了改变。
已建立了一个与诱导胶质瘤细胞分化相关的包含98个基因的基因表达谱。
