Davey M R, Gartland K M, Mulligan B J
Symp Soc Exp Biol. 1986;40:85-120.
Agrobacterium-induced transformation of plant cells results from integration of T-DNA of the Ti or Ri plasmids into the genome of susceptible plants. Expression of T-DNA genes induces physiological changes in transformed cells which modify normal plant development to produce proliferations characteristic of crown gall and hairy root diseases. Understanding of the molecular basis of the transformation events associated with these examples of naturally occurring genetic engineering of plant cells, has stimulated efforts to construct vectors for transferring specific genes into plants. Vector construction has progressed from the use of wild-type Ti plasmids, giving phenotypically abnormal regenerated plants, to non-oncogenic plasmids. The range of vectors now available should enable useful foreign genes to be inserted into a range of dicotyledons and monocotyledons without impairing normal plant development.
农杆菌诱导的植物细胞转化是由于Ti或Ri质粒的T-DNA整合到易感植物的基因组中。T-DNA基因的表达诱导转化细胞发生生理变化，这些变化改变了正常的植物发育，从而产生冠瘿和毛根病特有的增殖现象。对与这些植物细胞自然发生的基因工程实例相关的转化事件分子基础的理解，激发了构建用于将特定基因导入植物的载体的努力。载体构建已从使用野生型Ti质粒（产生表型异常的再生植物）发展到非致癌质粒。目前可用的载体范围应能使有用的外源基因插入到一系列双子叶植物和单子叶植物中，而不会损害植物的正常发育。
