在对干扰素无反应的工程化人类细胞中的病毒复制。
Virus replication in engineered human cells that do not respond to interferons.
作者信息
Young D F, Andrejeva L, Livingstone A, Goodbourn S, Lamb R A, Collins P L, Elliott R M, Randall R E
机构信息
School of Biology, University of St. Andrews, Fife KY16 9TS, United Kingdom.
出版信息
J Virol. 2003 Feb;77(3):2174-81. doi: 10.1128/jvi.77.3.2174-2181.2003.
Abstract
The V protein of the paramyxovirus simian virus 5 blocks interferon (IFN) signaling by targeting STAT1 for proteasome-mediated degradation. Here we report on the isolation of human cell lines that express the V protein and can no longer respond to IFN. A variety of viruses, particularly slow-growing wild-type viruses and vaccine candidate viruses (which are attenuated due to mutations that affect virus replication, virus spread, or ability to circumvent the IFN response), form bigger plaques and grow to titers that are increased as much as 10- to 4,000-fold in these IFN-nonresponsive cells. We discuss the practical applications of using such cells in vaccine development and manufacture, virus diagnostics and isolation of newly emerging viruses, and studies on host cell tropism and pathogenesis.
摘要
副粘病毒猴病毒5的V蛋白通过靶向信号转导和转录激活因子1（STAT1）进行蛋白酶体介导的降解来阻断干扰素（IFN）信号传导。在此，我们报告了表达V蛋白且不再对IFN作出反应的人细胞系的分离。多种病毒，特别是生长缓慢的野生型病毒和候选疫苗病毒（由于影响病毒复制、病毒传播或规避IFN反应能力的突变而减毒），在这些对IFN无反应的细胞中形成更大的噬斑，并且生长至滴度增加多达10至4000倍。我们讨论了使用此类细胞在疫苗开发与生产、病毒诊断及新出现病毒的分离，以及宿主细胞嗜性和发病机制研究中的实际应用。
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本文引用的文献
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The V proteins of simian virus 5 and other paramyxoviruses inhibit induction of interferon-beta.猿猴病毒5及其他副粘病毒的V蛋白可抑制β干扰素的诱导。
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