Bottomly H K, Perkins W D, Schwarz M R
J Immunol. 1975 Sep;115(3):648-52.
Tissue tolerance was induced in neonatal rats by the intravenous injection of bone marrow cells from adult allogeneic rat donors. After 6 to 8 weeks, lymphoid cells from rats in which tolerance had been induced were tested for mixed lymphocyte reactivity (MLR), 3H-uridine uptake, and the relationship of uridine incorporation to B and T lymphocytes. Lymph node (LN) and spleen (SPL) cells from the adult inoculated rats showed no reactivity in the MLR or normal lymphocyte transfer reaction (NLTRx), indicating that the animals were tolerant. After in vitro exposure to 3H-uridine, an abundance of small lymphocytes (SL) from these same tolerant rats were heavily labeled, in contrast to nontolerant controls, where relatively few SL were heavily labeled. In order to determine whether the heavily uridine-labeled cells were T cells or B cells, lymphoid cells from the LN and SPL of tolerant animals were exposed to either rabbit anti-AKR brain serum or rabbit anti-rat Ig conjugated with ferritin. The results showed that the heavily uridine-labeled SL of the tolerant rats were mainly Ig-positive cells.
通过静脉注射成年同种异体大鼠供体的骨髓细胞，在新生大鼠中诱导组织耐受性。6至8周后，对已诱导出耐受性的大鼠的淋巴细胞进行混合淋巴细胞反应（MLR）、3H-尿苷摄取以及尿苷掺入与B淋巴细胞和T淋巴细胞关系的检测。来自成年接种大鼠的淋巴结（LN）和脾脏（SPL）细胞在MLR或正常淋巴细胞转移反应（NLTRx）中无反应，表明这些动物具有耐受性。与未耐受的对照相比，在体外暴露于3H-尿苷后，来自这些相同耐受大鼠的大量小淋巴细胞（SL）被大量标记，而未耐受对照中只有相对较少的SL被大量标记。为了确定大量被尿苷标记的细胞是T细胞还是B细胞，将耐受动物的LN和SPL中的淋巴细胞暴露于兔抗-AKR脑血清或与铁蛋白偶联的兔抗大鼠Ig。结果显示，耐受大鼠中大量被尿苷标记的SL主要是Ig阳性细胞。
