Institute of Gastroenteropathy, Southwest Hospital, Third Military Medical University, Chongqing, China.
Eur J Cancer Prev. 2010 Sep;19(5):345-51. doi: 10.1097/CEJ.0b013e32833b4812.
Telomeres are the end structures of linear chromosomes in eukaryotic cells. The integrity of a telomere is essential for the overall stability of the chromosome. The human protection of telomeres 1 (hPOT1) protein, a single-stranded telomeric DNA binding protein, plays an important role in telomere protection and telomere length regulation. Here, we show that the loss of hPOT1 by RNA interference in BGC823 (poorly differentiated human gastric adenocarcinoma) cells leads to an increase in multinucleated giant cells, a decrease in cell proliferation and colony formation, induction of senescence and apoptosis, shortened telomere length, upregulation of the TRF1 gene and downregulation of the TRF2, tankyrase1 and hTERT genes. These results suggest that the loss of hPOT1 results in a decrease in the viability of BGC823 cells; hPOT1 regulates telomere length positively and has an influence on the expression of other telomere-associated genes in the cells.
端粒是真核细胞线性染色体的末端结构。端粒的完整性对于染色体的整体稳定性至关重要。人类端粒保护蛋白 1（hPOT1）是一种单链端粒 DNA 结合蛋白，在端粒保护和端粒长度调节中发挥重要作用。在这里，我们发现 RNA 干扰 BGC823（低分化人胃腺癌）细胞中的 hPOT1 表达缺失会导致多核巨细胞增加，细胞增殖和集落形成减少，衰老和凋亡诱导，端粒长度缩短，TRF1 基因上调，TRF2、端粒酶 1 和 hTERT 基因下调。这些结果表明，hPOT1 的缺失导致 BGC823 细胞活力下降；hPOT1 正向调节端粒长度，并影响细胞中端粒相关基因的表达。
