Nahalka Jozef, Liu Ziye, Chen Xi, Wang Peng George
Institute of Chemistry, Slovak Academy of Sciences Dúbravská cesta 9 SK-832 32 Bratislava, Slovak Republic.
Chemistry. 2003 Jan 20;9(2):372-7. doi: 10.1002/chem.200390038.
Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide regeneration cycles are necessary for the glycosylation. The multistep enzyme reaction can be efficiently carried out on superbeads that are prepared by immobilizing multienzyme mixtures on bead support through fused binding domains.
使用重组糖基转移酶进行酶促寡糖合成能够克服与化学方法相关的困难。尽管如此，糖基化反应仍需要糖核苷酸再生循环。通过将多酶混合物通过融合结合域固定在珠状载体上制备的超级珠，可以高效地进行多步酶反应。
