大豆苷元对雌激素受体阳性和阴性胰腺癌细胞的体外作用。
Effects of daidzein on estrogen-receptor-positive and negative pancreatic cancer cells in vitro.
作者信息
Guo Jun-Ming, Xiao Bing-Xiu, Dai De-Jian, Liu Qiong, Ma Hong-Hui
机构信息
School of Medicine, Ningbo University, Ningbo 315211, Zhejiang Province, China.
出版信息
World J Gastroenterol. 2004 Mar 15;10(6):860-3. doi: 10.3748/wjg.v10.i6.860.
Abstract
AIM
To study the effects of daidzein on human pancreatic cancer cells in vitro.
METHODS
Human estrogen-receptor (ER)-positive pancreatic cancer cells MiaPaCa-2 and ER-negative pancreatic cancer cells PANC-1 were treated by 0.1 micromol/L, 1 micromol/L, 10 microL, 25 microL, 50 microL, 75 microL and 100 microL of daidzein, respectively. Its antiproliferative effect was studied by MTT assay.
RESULTS
Daidzein inhibited the growth of MiaPaCa-2 and PANC-1 cells at the concentrations from 0.1 microL to 100 microL. A dose- and time-dependent manner was found. The IC(50) of daidzein on MiaPaCa-2 and PANC-1 cells was 45 microL and 75 micro, respectively. After MiaPaCa-2 cells were treated by daidzein for 3 d and at the concentrations more than IC(50), the inhibitory manner was identical and the inhibition appeared a saturation phenomenon, but the inhibitory manner of daidzein on PANC-1 cells was different from that of MiaPaCa-2 cells.
CONCLUSION
Daidzein has antiproliferative effects on human estrogen-receptor-positive and negative pancreatic cancer cells, but their mechanisms may be different.
摘要
目的
研究大豆苷元对人胰腺癌细胞的体外作用。
方法
分别用0.1微摩尔/升、1微摩尔/升、10微升、25微升、50微升、75微升和100微升的大豆苷元处理人雌激素受体（ER）阳性胰腺癌细胞MiaPaCa - 2和ER阴性胰腺癌细胞PANC - 1。通过MTT法研究其抗增殖作用。
结果
大豆苷元在0.1微升至100微升的浓度范围内抑制MiaPaCa - 2和PANC - 1细胞的生长。呈剂量和时间依赖性。大豆苷元对MiaPaCa - 2和PANC - 1细胞的IC（50）分别为45微升和75微升。大豆苷元以高于IC（50）的浓度处理MiaPaCa - 2细胞3天后，抑制方式相同且出现饱和现象，但大豆苷元对PANC - 1细胞的抑制方式与MiaPaCa - 2细胞不同。
结论
大豆苷元对人雌激素受体阳性和阴性胰腺癌细胞均有抗增殖作用，但其机制可能不同。
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