黑猩猩巨细胞病毒趋化因子vCXCL-1（CCMV）的功能特性
Functional characterization of chimpanzee cytomegalovirus chemokine, vCXCL-1(CCMV).
作者信息
Miller-Kittrell Mindy, Sai Jiqing, Penfold Mark, Richmond Ann, Sparer Tim E
机构信息
Department of Microbiology, University of Tennessee, Walters Life Sciences Rm F417, Knoxville, TN 37996, USA.
出版信息
Virology. 2007 Aug 1;364(2):454-65. doi: 10.1016/j.virol.2007.03.002. Epub 2007 Apr 11.
Abstract
Human cytomegaloviruses (HCMVs) are important pathogens in immunocompromised patients and newborns. The viral chemokine, vCXCL-1, of the Toledo (Tol) strain of HCMV has been implicated in HCMV virulence. Chimpanzee CMV (CCMV) has several genes with similarity to the vCXCL-1(Tol) gene, UL146. In order to test whether the CCMV viral chemokine, vCXCL-1(CCMV), is similar to vCXCL-1(Tol), we characterized its function in vitro. Receptor binding, activation, chemotaxis, signaling, and apoptosis in neutrophils were compared between vCXCL-1(Tol) and vCXCL-1(CCMV) and host chemokines. Although the homologues had similar activation potentials, chemotactic properties, and signaling, vCXCL-1(CCMV) had a approximately 70-fold lower affinity for CXCR2 and displayed differences in integrin upregulation and neutrophil apoptosis. These data demonstrate that in spite of extensive amino acid variability in vCXCL-1, CCMV may provide a model for assessing the role of vCXCL-1 in CMV pathogenesis in vivo.
摘要
人巨细胞病毒（HCMVs）是免疫功能低下患者和新生儿的重要病原体。HCMV托莱多（Tol）株的病毒趋化因子vCXCL-1与HCMV毒力有关。黑猩猩巨细胞病毒（CCMV）有几个与vCXCL-1（Tol）基因UL146相似的基因。为了测试CCMV病毒趋化因子vCXCL-1（CCMV）是否与vCXCL-1（Tol）相似，我们在体外对其功能进行了表征。比较了vCXCL-1（Tol）、vCXCL-1（CCMV）与宿主趋化因子在中性粒细胞中的受体结合、激活、趋化性、信号传导和凋亡情况。尽管同源物具有相似的激活潜能、趋化特性和信号传导，但vCXCL-1（CCMV）对CXCR2的亲和力低约70倍，并且在整合素上调和中性粒细胞凋亡方面存在差异。这些数据表明，尽管vCXCL-1存在广泛的氨基酸变异性，但CCMV可能为评估vCXCL-1在体内CMV发病机制中的作用提供一个模型。
相似文献
1
Functional characterization of chimpanzee cytomegalovirus chemokine, vCXCL-1(CCMV).黑猩猩巨细胞病毒趋化因子vCXCL-1（CCMV）的功能特性
Virology. 2007 Aug 1;364(2):454-65. doi: 10.1016/j.virol.2007.03.002. Epub 2007 Apr 11.
2
Novel Human Cytomegalovirus Viral Chemokines, vCXCL-1s, Display Functional Selectivity for Neutrophil Signaling and Function.新型人类巨细胞病毒病毒趋化因子vCXCL-1s对中性粒细胞信号传导和功能具有功能选择性。
J Immunol. 2015 Jul 1;195(1):227-36. doi: 10.4049/jimmunol.1400291. Epub 2015 May 18.
3
Expression of human CXCR2 in murine neutrophils as a model for assessing cytomegalovirus chemokine vCXCL-1 function in vivo.人CXCR2在鼠中性粒细胞中的表达作为体内评估巨细胞病毒趋化因子vCXCL-1功能的模型。
J Interferon Cytokine Res. 2004 Oct;24(10):611-20. doi: 10.1089/jir.2004.24.611.
4
The Human Cytomegalovirus Chemokine vCXCL-1 Modulates Normal Dissemination Kinetics of Murine Cytomegalovirus .人类巨细胞病毒趋化因子 vCXCL-1 调节小鼠巨细胞病毒的正常扩散动力学。
mBio. 2019 Jun 25;10(3):e01289-19. doi: 10.1128/mBio.01289-19.
5
The frequency of cytomegalovirus non-ELR UL146 genotypes in neonates with congenital CMV disease is comparable to strains in the background population.巨细胞病毒非 ELR UL146 基因型在先天性巨细胞病毒病新生儿中的频率与背景人群中的菌株相当。
BMC Infect Dis. 2021 Apr 26;21(1):386. doi: 10.1186/s12879-021-06076-w.
6
A little cooperation helps murine cytomegalovirus (MCMV) go a long way: MCMV co-infection rescues a chemokine salivary gland defect.一点合作能助力小鼠巨细胞病毒（MCMV）走得更远：MCMV共感染挽救了趋化因子唾液腺缺陷。
J Gen Virol. 2016 Nov;97(11):2957-2972. doi: 10.1099/jgv.0.000603. Epub 2016 Sep 13.
7
Sequence variability of human cytomegalovirus UL146 and UL147 genes in low-passage clinical isolates.低传代临床分离株中人巨细胞病毒UL146和UL147基因的序列变异性
Intervirology. 2006;49(4):215-23. doi: 10.1159/000091468. Epub 2006 Feb 16.
8
The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome.人类巨细胞病毒基因组再探：与黑猩猩巨细胞病毒基因组的比较
J Gen Virol. 2003 Jan;84(Pt 1):17-28. doi: 10.1099/vir.0.18606-0.
9
Cytomegalovirus encodes a potent alpha chemokine.巨细胞病毒编码一种强效α趋化因子。
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9839-44. doi: 10.1073/pnas.96.17.9839.
10
Fatal attraction: cytomegalovirus-encoded chemokine homologs.致命诱惑：巨细胞病毒编码的趋化因子同源物
Curr Top Microbiol Immunol. 2002;269:235-56. doi: 10.1007/978-3-642-59421-2_14.
引用本文的文献
1
The Human Cytomegalovirus Chemokine vCXCL-1 Modulates Normal Dissemination Kinetics of Murine Cytomegalovirus .人类巨细胞病毒趋化因子 vCXCL-1 调节小鼠巨细胞病毒的正常扩散动力学。
mBio. 2019 Jun 25;10(3):e01289-19. doi: 10.1128/mBio.01289-19.
2
New extracellular factors in glioblastoma multiforme development: neurotensin, growth differentiation factor-15, sphingosine-1-phosphate and cytomegalovirus infection.多形性胶质母细胞瘤发展中的新细胞外因子：神经降压素、生长分化因子-15、鞘氨醇-1-磷酸与巨细胞病毒感染。
Oncotarget. 2018 Jan 9;9(6):7219-7270. doi: 10.18632/oncotarget.24102. eCollection 2018 Jan 23.
3
Human Cytomegalovirus Delays Neutrophil Apoptosis and Stimulates the Release of a Prosurvival Secretome.人巨细胞病毒延迟中性粒细胞凋亡并刺激促生存分泌组的释放。
Front Immunol. 2017 Sep 25;8:1185. doi: 10.3389/fimmu.2017.01185. eCollection 2017.
4
Chemokines encoded by herpesviruses.疱疹病毒编码的趋化因子。
J Leukoc Biol. 2017 Nov;102(5):1199-1217. doi: 10.1189/jlb.4RU0417-145RR. Epub 2017 Aug 28.
5
A little cooperation helps murine cytomegalovirus (MCMV) go a long way: MCMV co-infection rescues a chemokine salivary gland defect.一点合作能助力小鼠巨细胞病毒（MCMV）走得更远：MCMV共感染挽救了趋化因子唾液腺缺陷。
J Gen Virol. 2016 Nov;97(11):2957-2972. doi: 10.1099/jgv.0.000603. Epub 2016 Sep 13.
6
Bioactive Molecules Released From Cells Infected with the Human Cytomegalovirus.从感染人巨细胞病毒的细胞中释放的生物活性分子。
Front Microbiol. 2016 May 13;7:715. doi: 10.3389/fmicb.2016.00715. eCollection 2016.
7
Novel Human Cytomegalovirus Viral Chemokines, vCXCL-1s, Display Functional Selectivity for Neutrophil Signaling and Function.新型人类巨细胞病毒病毒趋化因子vCXCL-1s对中性粒细胞信号传导和功能具有功能选择性。
J Immunol. 2015 Jul 1;195(1):227-36. doi: 10.4049/jimmunol.1400291. Epub 2015 May 18.
8
NF-κB and STAT1 control CXCL1 and CXCL2 gene transcription.NF-κB 和 STAT1 控制 CXCL1 和 CXCL2 基因转录。
Am J Physiol Endocrinol Metab. 2014 Jan 15;306(2):E131-49. doi: 10.1152/ajpendo.00347.2013. Epub 2013 Nov 26.
9
HCMV-encoded UL128 enhances TNF-α and IL-6 expression and promotes PBMC proliferation through the MAPK/ERK pathway in vitro.巨细胞病毒编码的 UL128 通过 MAPK/ERK 通路在体外增强 TNF-α 和 IL-6 的表达并促进 PBMC 增殖。
Viral Immunol. 2012 Apr;25(2):98-105. doi: 10.1089/vim.2011.0064.
10
How the virus outsmarts the host: function and structure of cytomegalovirus MHC-I-like molecules in the evasion of natural killer cell surveillance.病毒如何智胜宿主：巨细胞病毒MHC-I类分子在逃避自然杀伤细胞监测中的功能与结构
J Biomed Biotechnol. 2011;2011:724607. doi: 10.1155/2011/724607. Epub 2011 Jun 30.
本文引用的文献
1
Cytomegalovirus (CMV)-encoded UL144 (truncated tumor necrosis factor receptor) and outcome of congenital CMV infection.巨细胞病毒（CMV）编码的UL144（截短的肿瘤坏死因子受体）与先天性CMV感染的结局
J Infect Dis. 2006 Aug 15;194(4):464-73. doi: 10.1086/505427. Epub 2006 Jul 7.
2
Sequence variability of human cytomegalovirus UL146 and UL147 genes in low-passage clinical isolates.低传代临床分离株中人巨细胞病毒UL146和UL147基因的序列变异性
Intervirology. 2006;49(4):215-23. doi: 10.1159/000091468. Epub 2006 Feb 16.
3
Human cytomegalovirus-encoded alpha -chemokines exhibit high sequence variability in congenitally infected newborns.人巨细胞病毒编码的α趋化因子在先天性感染的新生儿中表现出高度的序列变异性。
J Infect Dis. 2006 Mar 15;193(6):788-91. doi: 10.1086/500508. Epub 2006 Feb 8.
4
Analysis of the human cytomegalovirus genomic region from UL146 through UL147A reveals sequence hypervariability, genotypic stability, and overlapping transcripts.对人巨细胞病毒从UL146至UL147A的基因组区域分析显示出序列高度变异性、基因型稳定性及重叠转录本。
Virol J. 2006 Jan 12;3:4. doi: 10.1186/1743-422X-3-4.
5
Contribution of GADD45 family members to cell death suppression by cellular Bcl-xL and cytomegalovirus vMIA.GADD45家族成员对细胞Bcl-xL和巨细胞病毒vMIA抑制细胞死亡的作用。
J Virol. 2005 Dec;79(23):14923-32. doi: 10.1128/JVI.79.23.14923-14932.2005.
6
Mitochondrial cell death suppressors carried by human and murine cytomegalovirus confer resistance to proteasome inhibitor-induced apoptosis.人类和鼠类巨细胞病毒携带的线粒体细胞死亡抑制因子赋予对蛋白酶体抑制剂诱导的细胞凋亡的抗性。
J Virol. 2005 Oct;79(19):12205-17. doi: 10.1128/JVI.79.19.12205-12217.2005.
7
Alanine scanning mutagenesis of the chemokine receptor CCR3 reveals distinct extracellular residues involved in recognition of the eotaxin family of chemokines.趋化因子受体CCR3的丙氨酸扫描诱变揭示了参与识别嗜酸性粒细胞趋化因子家族趋化因子的不同细胞外残基。
Mol Immunol. 2006 Mar;43(8):1221-31. doi: 10.1016/j.molimm.2005.07.015. Epub 2005 Aug 15.
8
Chemokine receptor internalization and intracellular trafficking.趋化因子受体内化与细胞内运输。
Cytokine Growth Factor Rev. 2005 Dec;16(6):637-58. doi: 10.1016/j.cytogfr.2005.05.008. Epub 2005 Jul 5.
9
Loss of linkage disequilibrium and accelerated protein divergence in duplicated cytomegalovirus chemokine genes.巨细胞病毒趋化因子基因复制过程中连锁不平衡的丧失与蛋白质分化加速
Virus Genes. 2005 Aug;31(1):65-72. doi: 10.1007/s11262-005-2201-3.
10
Neutrophil beta2 integrins: moderators of life or death decisions.中性粒细胞β2整合素：生死抉择的调节因子
Trends Immunol. 2005 Jul;26(7):388-95. doi: 10.1016/j.it.2005.05.002.
Zotero 插件