两种复合PCR系统对广东汉族人群13个基因座的遗传研究及其法医学应用
[Genetic studies of 13 loci in Guangdong Han population by two multiplex PCR systems and its forensic application].
作者信息
Li Y, Wang S B, Liu C, Li H X, Hu H Y, Liu H, Liu C H, Chen X H
机构信息
Guangzhou Criminal Science & Technology Institute, Guangzhou 510030.
出版信息
Fa Yi Xue Za Zhi. 2001 May;17(2):82-5.
OBJECTIVE
The genetic studies of 13 short tandem repeats(STRs) loci in two multiplex amplification systems were carried out on Chinese Han population in Guangdong.
METHODS
DNA samples from 328 unrelated individuals were screened. The 13 loci were D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, TH01, TPOX and CSF1PO. The PCR products were analyzed and genotyped by ABI 377-96 Sequencer.
RESULTS
The combined power of discrimination (DP) was 0.999999999999993 and the combined paternity of exclusion(PE) was 99.999%. These 13 loci met the Hardy-Weinberg expectations.
CONCLUSION
The two multiplex amplification systems were very useful in forensic case investigation.
目的
对广东汉族人群两个复合扩增系统中的13个短串联重复序列（STRs）位点进行遗传学研究。
方法
筛选328名无关个体的DNA样本。这13个位点分别为D3S1358、vWA、FGA、D8S1179、D21S11、D18S51、D5S818、D13S317、D7S820、D16S539、TH01、TPOX和CSF1PO。PCR产物经ABI 377 - 96测序仪分析并进行基因分型。
结果
累积个体识别力（DP）为0.999999999999993，累积非父排除率（PE）为99.999%。这13个位点均符合哈迪 - 温伯格平衡。
结论
这两个复合扩增系统在法医案件调查中非常有用。
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