Chen Ling, Lu Hui-Jie, DU Wei-An, Qiu Ping-Ming, Liu Chao
School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2016 Feb 20;37(2):141-149. doi: 10.3969/j.issn.1673-4254.2017.02.01.
To evaluate the value of PowerPlex21 System (Promega) and study the genetic polymorphism of its 20 short-tandem repeat (STR) loci in southern Chinese Han population.
We conducted genotyping experiments using PowerPlex21 System on 20 autosomal STR loci (D3S1358, D1S1656, D6S1043, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433 and FGA) in 2367 unrelated Chinese Han individuals living in South China. The allele frequencies and parameters commonly used in forensic science were statistically analyzed in these individuals and compared with the reported data of other populations.
The PowerPlex 21 System had a power of discrimination (PD) ranging from 0.7839 to 0.9852 and a power of exclusion (PE) ranging from 0.2974 to 0.8099 for the 20 loci. No significant deviation from Hardy-Weinberg expectations was found for all the loci except for D5S818. This southern Chinese Han population had significant differences in the allele frequencies from 8 ethnic groups reported in China, and showed significant differences at 8 to 20 STR foci from 5 foreign populations. The allele frequency at the locus D1S1656 in this southern Chinese Han population differed significantly from those in the 5 foreign populations and from 3 reported Han populations in Beijing, Zhejiang Province and Fujian Province of China. The neighbor-joining phylogenetictree showed clustering of all the Asian populations in one branch, while the northern Italian and Argentina populations clustered in a separate branch. This southern Chinese Han population had the nearest affinity with the Yi ethnic population in Yunnan Province of China.
The 20 STR loci are highly polymorphic in this southern Chinese Han population, suggesting the value of this set of STR loci in forensic personal identification, paternity testing and anthropological study.
评估PowerPlex21系统（普洛麦格公司）的价值，并研究其20个短串联重复序列（STR）基因座在中国南方汉族人群中的遗传多态性。
我们使用PowerPlex21系统对2367名居住在中国南方的无血缘关系的汉族个体的20个常染色体STR基因座（D3S1358、D1S1656、D6S1043、D13S317、五核苷酸E、D16S539、D18S51、D2S1338、CSF1PO、五核苷酸D、TH01、vWA、D21S11、D7S820、D5S818、TPOX、D8S1179、D12S391、D19S433和FGA）进行基因分型实验。对这些个体中法医科学常用的等位基因频率和参数进行统计分析，并与其他人群的报告数据进行比较。
PowerPlex 21系统对这20个基因座的鉴别力（PD）范围为0.7839至0.9852，排除力（PE）范围为0.2974至0.8099。除D5S818外，所有基因座均未发现与哈迪-温伯格预期有显著偏差。该中国南方汉族人群与中国报道的8个民族的等位基因频率存在显著差异，与5个外国人群在8至20个STR位点上存在显著差异。该中国南方汉族人群中D1S1656基因座的等位基因频率与5个外国人群以及中国北京、浙江和福建报道的3个汉族人群的等位基因频率存在显著差异。邻接法系统发育树显示所有亚洲人群聚集在一个分支中，而意大利北部和阿根廷人群聚集在另一个分支中。该中国南方汉族人群与中国云南省的彝族人群亲缘关系最近。
这20个STR基因座在中国南方汉族人群中具有高度多态性，表明这组STR基因座在法医个人识别、亲子鉴定和人类学研究中的价值。
