基于湖南省汉族人群的21个STR基因座的遗传多态性
Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population.
作者信息
Zou Y, Guo J J, Li Q P, Zuo D H, Liu J S, Guo Y D, Yan J, Zha L, Cai J F, Lan L M
机构信息
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Xiangya School of Medicine, Central South University, Changsha 410013, China.
出版信息
Fa Yi Xue Za Zhi. 2016 Oct;32(5):356-362. doi: 10.3969/j.issn.1004-5619.2016.05.010. Epub 2016 Oct 25.
OBJECTIVES
To investigate the genetic polymorphisms of 21 short tandem repeat （STR） loci （, , , , , , , , , , , , , , , , , , , and ）.
METHODS
A total of 560 blood samples were collected from unrelated healthy individuals of Han population in Hunan Province. Chelex-100 extraction method was applied to the extraction of genomic DNA, and an AGCU EX22 Kit and 9700 STR amplification was used in amplification reactions. The products were separated and analyzed on 310 Genetic Analyzer.
RESULTS
A total of 248 alleles were observed, the allelic frequencies ranging from 0.001 to 0.518. Observation of genotype distributions for each locus showed no deviations from Hardy-Weinberg equilibrium except （=0.023）. The combined power of discrimination, combined power of exclusion, and combined matching probability of the 21 STR loci were approximately 0.999 999 999 999 999 999 999 999 8, 0.999 999 998, and 1.36×10⁻²⁵, respectively.
CONCLUSIONS
The 21 STR loci show high polymorphisms in the Han population, which can provide valuable data and a theoretical basis for forensic individual identification and paternity testing.
目的
研究21个短串联重复序列（STR）基因座（, , , , , , , , , , , , , , , , , , , 及 ）的基因多态性。
方法
从湖南省汉族无关健康个体中采集560份血液样本。采用Chelex-100提取法提取基因组DNA，使用AGCU EX22试剂盒及9700进行STR扩增反应。产物在310遗传分析仪上进行分离和分析。
结果
共观察到248个等位基因，等位基因频率范围为0.001至0.518。各基因座基因型分布观察结果显示，除 （=0.023）外，均未偏离哈迪-温伯格平衡。21个STR基因座的联合鉴别力、联合排除力及联合匹配概率分别约为0.999 999 999 999 999 999 999 999 8、0.999 999 998及1.36×10⁻²⁵。
结论
21个STR基因座在汉族人群中显示出高度多态性，可为法医个体识别和亲子鉴定提供有价值的数据及理论依据。
Zotero 插件