Stornelli A, Arauz M, Baschard H, de la Sota R-L
Institute of Theriogenology and Central Laboratory, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Argentina.
Reprod Domest Anim. 2003 Feb;38(1):1-4. doi: 10.1046/j.1439-0531.2003.00369.x.
The objective of this research was to use a model of unilateral and bilateral occlusion of the ductus deferens in the dog to study the use of alkaline phosphatase (AP) as an indicator of tubular patency. Seven healthy cross bred dogs weighing 10-15 kg BW with normal spermiogram and AP concentrations in semen were used. From each dog, three semen samples were obtained before (intact) and after right (unilateral) and left (bilateral) vasectomy. The AP concentrations were measured in duplicates by a colorimetric method in each of the three fractions (first, second (sperm-rich), third) of each ejaculate. In addition, a macroscopic and microscopic evaluation of each ejaculate was carried out to assure its quality. Data were analysed by least squares analysis of variance using SAS(R). In intact and unilateral vasectomized dogs, 96.6% of AP measured in semen corresponded to the second sperm-rich fraction whereas 1.53 and 1.83% corresponded to the first and third fractions respectively. Total AP concentrations (first and second and third fraction) in vasectomized dogs were lower than in intact animals (19.857 vs 2284.431 +/- 4.347 UAL; p < 0.001). AP concentrations were much lower in bilateral than in unilateral vasectomized dogs (142 vs 39.572 +/- 4.347 UL, p < 0.001). In summary, AP concentrations in semen can be used as an early indicator of unilateral or bilateral lack of patency of the epididymal and deferent ducts in the dog.
本研究的目的是利用犬单侧和双侧输精管结扎模型，研究将碱性磷酸酶（AP）用作管道通畅性指标的情况。选用了7只体重10 - 15千克、精液图谱和精液中AP浓度正常的健康杂种犬。从每只犬身上，在右侧（单侧）和左侧（双侧）输精管结扎术前（完整状态）和术后获取三份精液样本。对每份射精的三个部分（第一部分、第二部分（富含精子部分）、第三部分）中的AP浓度采用比色法进行双份测量。此外，对每份射精进行宏观和微观评估以确保其质量。使用SAS(R)通过最小二乘方差分析对数据进行分析。在完整和单侧输精管结扎的犬中，精液中测得的AP有96.6%对应于富含精子的第二部分，而分别有1.53%和1.83%对应于第一部分和第三部分。输精管结扎犬的总AP浓度（第一、第二和第三部分）低于完整动物（19.857对2284.431±4.347 UAL；p<0.001）。双侧输精管结扎犬的AP浓度比单侧输精管结扎犬低得多（142对39.572±4.347 UL，p<0.001）。总之，精液中的AP浓度可用作犬附睾和输精管单侧或双侧不通畅的早期指标。
