Wu H J, Shen M, Xian P, Xiang P, Shen B H, Bu J, Huang Z J
Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, P. R. China.
Fa Yi Xue Za Zhi. 1999 May;15(2):93-4, 125.
This article describes a sensitive method that detects morphine, 6-monoacetylmorphine, morphine-3-glucuronide and codeine in urine for qualifying the abuse of heroin. The analytes were extracted by solid phase C18. The limits of detection (LOD) for morphine and codeine were 50 ng/ml and 50 ng/ml, respectively. The RSD of morphine and codeine were 11.3% (n = 5), and 14.2% (n = 5) respectively. For urine, it does not need to be hydrolyzed before extracted, and for all analytes, also need not to be derivated. The difference ratio of morphine and codeine in the chromatography can be used to discriminate between the abuse of heroin and the administration of compound liquorice mixture.
本文描述了一种灵敏的方法，可检测尿液中的吗啡、6-单乙酰吗啡、吗啡-3-葡萄糖醛酸苷和可待因，以鉴定海洛因滥用情况。分析物通过固相C18萃取。吗啡和可待因的检测限（LOD）分别为50 ng/ml和50 ng/ml。吗啡和可待因的相对标准偏差（RSD）分别为11.3%（n = 5）和14.2%（n = 5）。对于尿液，萃取前无需水解，对于所有分析物，也无需衍生化。色谱图中吗啡和可待因的差异比可用于区分海洛因滥用和复方甘草合剂的使用。
