Vu-Duc T, Vernay A
Institute of Occupational Health Sciences, Unit of Drug Analysis, Lausanne, Switzerland.
Biomed Chromatogr. 1990 Mar;4(2):65-9. doi: 10.1002/bmc.1130040206.
It is of importance to differentiate heroin intake from the absorption of opiate-containing pharmaceuticals or opiates from other sources. A method for the routine determination of O6-monoacetylmorphine (6-MAM), the specific metabolite of heroin in human urine, by gas chromatography and classical detectors without having recourse to gas chromatography/mass spectrometry-selected ion mode (GC/MS-SIM) is described. With dual detection by nitrogen selective and flame ionization detectors, the limits of detection for 6-MAM were determined to be 2 ng/mL and 4 ng/mL urine for a 10 mL sample. When applied to urines preliminarily screened for opiates, the results appeared consistent in comparison with those obtained by GC/MS-SIM. The method was also developed for the simultaneous quantitative analysis of morphine and codeine. The linearity was tested up to 600 ng/mL for the three compounds of interest 6-MAM, morphine and codeine and their absolute recoveries were 76%, 78%, 75% respectively.
区分海洛因摄入与含阿片类药物或其他来源阿片的吸收情况很重要。本文描述了一种通过气相色谱法和传统检测器常规测定人尿中 heroin 特异性代谢物 O6 - 单乙酰吗啡（6 - MAM）的方法，无需借助气相色谱/质谱 - 选择离子模式（GC/MS - SIM）。采用氮选择性检测器和火焰离子化检测器进行双重检测，对于 10 mL 尿液样品，6 - MAM 的检测限分别确定为 2 ng/mL 和 4 ng/mL 尿液。当应用于初步筛选过阿片类药物的尿液时，与 GC/MS - SIM 获得的结果相比，结果似乎一致。该方法还用于吗啡和可待因的同时定量分析。对感兴趣的三种化合物 6 - MAM、吗啡和可待因进行线性测试，最高可达 600 ng/mL，它们的绝对回收率分别为 76%、78%、75%。
