Zhou M, Zhong H J, Wu X Y
Department of Endocrinology, Xiangya Hospital, Central South University, Changsha 410008.
Hunan Yi Ke Da Xue Xue Bao. 2001 Feb 28;26(1):41-4.
To investigate the alteration of ultrastructure and function of the aortic endothelium in streptozotocin (STZ)-diabetic rats and the effect of perindopril, male SD rats were randomly divided into normal group (NC), diabetes control group (DC), diabetes group treated with perindopril (2 mg.kg-1.d-1) which was administered after 4 weeks. At 4, 8, 16 weeks after injecting STZ, glucose, plasma endothelin-1 and angiotensin II were respectively measured, and we also observed aortic endothelial cell under the electron microscope.
In DC group, there were mitochondrial edema and vacuolization obviously in aortic endothelial cells at 8 weeks, and extensive endothelial cell necrosis and exfoliation were observed at 16 weeks, while pathological changes in the DP group were abated significantly. Plasma Ang II levels were increased significantly at different times in DC group, and plasma ET-1 levels were obviously increased at 8 weeks and greatly decreased at 16 weeks. In DP groups, plasma Ang II levels obviously decreased, ET-1 levels declined at 8 weeks and statistically elevated at 16 weeks.
There are changes of ultrastructure and function in aortic endothelial cell in different durations of diabetes. Plasma ET-1 level may be a marker of aortic endothelial cell injury, perindopril may have protective effect on aortic endothelial cell in diabetic rats.
为研究链脲佐菌素（STZ）诱导的糖尿病大鼠主动脉内皮超微结构和功能的改变以及培哚普利的作用，将雄性SD大鼠随机分为正常组（NC）、糖尿病对照组（DC）、糖尿病培哚普利治疗组（4周后给予2mg·kg-1·d-1培哚普利）。注射STZ后4、8、16周分别检测血糖、血浆内皮素-1和血管紧张素II，并在电镜下观察主动脉内皮细胞。
DC组8周时主动脉内皮细胞线粒体明显水肿、空泡化，16周时可见广泛的内皮细胞坏死、剥脱，而DP组病理改变明显减轻。DC组不同时间血浆Ang II水平均显著升高，8周时血浆ET-1水平明显升高，16周时大幅下降。DP组血浆Ang II水平明显降低，8周时ET-1水平下降，16周时统计学上升高。
糖尿病不同病程中主动脉内皮细胞存在超微结构和功能改变。血浆ET-1水平可能是主动脉内皮细胞损伤的标志物，培哚普利对糖尿病大鼠主动脉内皮细胞可能有保护作用。
